{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T16:09:44Z","timestamp":1774714184271,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T00:00:00Z","timestamp":1533772800000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Porphyromonas gingivalis<\/jats:italic> lipopolysaccharide (LPS) plays a major role in the initiation and progression of chronic periodontitis. Human gingival fibroblasts (HGFs) interact with bacteria or bacterial products and trigger inflammatory signaling pathways that destroy periodontal tissues. RhoA regulates cytokine production in various cell types. This study investigated the role of Rho\u2010kinase inhibitor Y\u201027632 in LPS\u2010induced nuclear factor\u2010kappa B (NF\u2010\u03baB) and p\u201038 mitogen\u2010activated protein kinase (MAPK) activation, and inflammatory cytokine production in HGFs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Effects of Y\u201027632, SB203580 (p38 MAPK inhibitor), and BAY11\u20107082 (NF\u2010\u03baB inhibitor) were assessed in lipopolysaccharide (LPS)\u2010treated HGFs. Cytotoxicity assays were used to determine the effect of the drugs on HGF viability. Enzyme\u2010linked immunosorbent assays and quantitative real\u2010time polymerase chain reaction were applied to evaluate the levels of interleukin (IL)\u20106, IL\u20108, and Toll\u2010like receptors (TLRs). NF\u2010\u03baB and p38 MAPK pathway activation was detected by western blot and immunocytochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>P. gingivalis<\/jats:italic> LPS at 5\u00a0\u03bcg\/mL, 10\u00a0\u03bcM Y\u201027632, 10\u00a0\u03bcM SB203580, and 5\u00a0\u03bcM BAY11\u20107082 exhibited no toxicity in HGFs. LPS activated NF\u2010\u03baB and p38 MAPK by increasing degradation of I\u03baB\u03b1 and phosphorylation of I\u03baB\u03b1, p65, and p38, and facilitating p65 translocation from the cytoplasm to nuclei. The activation of NF\u2010\u03baB and p38 MAPK induced overproduction of IL\u20106 and IL\u20108 at both mRNA and protein levels. However, Y\u201027632 attenuated LPS\u2010induced NF\u2010\u03baB and p38 MAPK activation and inflammatory cytokine production.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Rho\u2010kinase inhibitor Y\u201027632 downregulates LPS\u2010induced IL\u20106 and IL\u20108 production by blocking NF\u2010\u03baB and p38 MAPK activation in HGFs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.17-0571","type":"journal-article","created":{"date-parts":[[2018,5,4]],"date-time":"2018-05-04T03:51:13Z","timestamp":1525405873000},"page":"883-893","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Rho\u2010kinase inhibitor Y\u201027632 downregulates LPS\u2010induced IL\u20106 and IL\u20108 production via blocking p38 MAPK and NF\u2010\u03baB pathways in human gingival fibroblasts"],"prefix":"10.1002","volume":"89","author":[{"given":"Wenyan","family":"Kang","sequence":"first","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology, Shandong University  Shandong Jinan China"},{"name":"Department of Periodontology School of Stomatology, Shandong University  Shandong Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingling","family":"Shang","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology, Shandong University  Shandong Jinan China"},{"name":"Department of Periodontology School of Stomatology, Shandong University  Shandong Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology, Shandong University  Shandong Jinan 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