{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:21:45Z","timestamp":1790392905527,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T00:00:00Z","timestamp":1534204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Non\u2010alcoholic steatohepatitis (NASH) is one of the chronic liver diseases that can develop into hepatocirrhosis. The purpose of the present study was to investigate the impact of lipopolysaccharide (LPS) from <jats:italic>Porphyromonas gingivalis (P. gingivalis)<\/jats:italic> on NASH onset, and to determine the biodistribution of double\u2010radiolabeled LPS (R\u2010LPS) biosynthesized in <jats:italic>P. gingivalis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rats fed a basal diet (BD) or a high\u2010fat diet (HD) were injected with <jats:italic>P. gingivalis<\/jats:italic>\u2010LPS or R\u2010LPS into the palatine gingiva around the right maxillary first molar, and were classified into the following 4 groups: BD\/LPS (\u2010), BD\/LPS (+), HD\/LPS (\u2010), and HD\/LPS (+) or 2 groups: BD\/R\u2010LPS and HD\/R\u2010LPS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Inflammation in the gingiva of the LPS (+) groups progressed significantly more than the LPS (\u2010) groups. Furthermore, in the HD\/LPS (+) liver, histologic analysis confirmed the presence of NASH, characterized by large fat droplets, ballooning degeneration, and infiltration of inflammatory cells. When <jats:sup>3<\/jats:sup>H, <jats:sup>14<\/jats:sup>C\u2010R\u2010LPS was injected into the palatine gingiva, radioactivity in the right palatal mucosa of HD\/R\u2010LPS rats was the highest in comparison with other regions and was significantly elevated after 24\u00a0hours compared to BD\/R\u2010LPS rats. Autoradiographic analysis of the maxilla showed distributions from the palatal mucosa to the hard palate and the interdental region. Radioactivity in organs of both BD\/R\u2010LPS and HD\/R\u2010LPS rats were mostly localized to the liver even after 24\u00a0hours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present study suggests that the transfer of <jats:italic>P. gingivalis<\/jats:italic>\u2010LPS from the oral cavity to the liver plays an important role in disease exacerbation of NASH.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.17-0678","type":"journal-article","created":{"date-parts":[[2018,5,25]],"date-time":"2018-05-25T09:29:01Z","timestamp":1527240541000},"page":"1101-1111","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Histological effects and pharmacokinetics of lipopolysaccharide derived from <i>Porphyromonas gingivalis<\/i> on rat maxilla and liver concerning with progression into non\u2010alcoholic steatohepatitis"],"prefix":"10.1002","volume":"89","author":[{"given":"Miyako","family":"Fujita","sequence":"first","affiliation":[{"name":"Department of Periodontology The Nippon Dental University School of Life Dentistry at Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryutaro","family":"Kuraji","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Nippon Dental University School of Life Dentistry at Tokyo  Tokyo Japan"},{"name":"Department of Life Science Dentistry The Nippon Dental University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Nippon Dental University School of Life Dentistry at Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"The Nippon Dental University  Tokyo 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