{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:06:54Z","timestamp":1785557214264,"version":"3.56.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T00:00:00Z","timestamp":1535587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP25463218"],"award-info":[{"award-number":["JP25463218"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recently, brain\u2010derived neurotrophic factor (BDNF)\/high molecular weight hyaluronic acid (HMW\u2010HA) complex with flap surgery has been shown to promote periodontal tissue regeneration. The objective of this study was to evaluate the effects of local subgingival application of BDNF\/HMW\u2010HA complex adjunctive to scaling and root planning (SRP) on ligature\u2010induced periodontitis in dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The dogs were divided into four treatment groups: no treatment (control), SRP alone, SRP followed by local application of HMW\u2010HA (SRP+HMW\u2010HA), and SRP followed by local application of BDNF (500\u00a0\u03bcg\/ml)\/ HMW\u2010HA complex (SRP+BDNF\/HMW\u2010HA). HMW\u2010HA or BDNF\/HMW\u2010HA complex was topically applied to periodontal pockets using a syringe without surgery. Two weeks after treatment, clinical parameters (gingival index, clinical attachment level, periodontal pocket depth and bleeding on probing) were recorded and specimens were collected from anesthetized animals for histological analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The SRP+BDNF\/HMW\u2010HA group showed significant improvement in all clinical parameters compared to other treatment groups. Histologic analysis showed greater suppression of apical migration of epithelial tissue and milder inflammatory cell infiltration in the SRP+BDNF\/HMW\u2010HA group than in the other treatment groups. Furthermore, new cementum and alveolar bone were regenerated, and collagen fibers were inserted into them in the SRP+BDNF\/HMW\u2010HA group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>BDNF\/HMW\u2010HA complex as an adjunct to nonsurgical periodontal treatment has the potential to reduce excess inflammation. Further investigation will be needed to clarify periodontal tissue regenerative effects of BDNF\/HMW\u2010HA complex in a nonsurgical setting.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.18-0070","type":"journal-article","created":{"date-parts":[[2018,7,21]],"date-time":"2018-07-21T02:54:12Z","timestamp":1532141652000},"page":"98-109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["BDNF\/HMW\u2010HA complex as an adjunct to nonsurgical periodontal treatment of ligature\u2010induced periodontitis in dogs"],"prefix":"10.1002","volume":"90","author":[{"given":"Shinya","family":"Sasaki","sequence":"first","affiliation":[{"name":"Department of Periodontal Medicine Graduate school of Biomedical &amp; Health Sciences Hiroshima University  Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiro","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Periodontal 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