{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:14:14Z","timestamp":1790403254790,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,8,21]],"date-time":"2018-08-21T00:00:00Z","timestamp":1534809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recent findings about the differential gene expression signature of periodontal lesions have raised the hypothesis of distinctive biological phenotypes expressed by generalized chronic periodontitis (GCP) and generalized aggressive periodontitis (GAgP) patients. Therefore, this cross\u2010sectional investigation was planned, primarily, to determine the ability of nuclear magnetic resonance (NMR) spectroscopic analysis of unstimulated whole saliva to discriminate GCP and GAgP disease\u2010specific metabolomic fingerprint and, secondarily, to assess potential metabolites discriminating periodontitis patients from periodontally healthy individuals (HI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>NMR\u2010metabolomics spectra were acquired from salivary samples of patients with a clinical diagnosis of GCP (<jats:italic>n<\/jats:italic>\u00a0=\u00a033) or GAgP (<jats:italic>n<\/jats:italic>\u00a0=\u00a028) and from HI (<jats:italic>n<\/jats:italic>\u00a0=\u00a039). The clustering of HI, GCP, and GAgP patients was achieved by using a combination of the Principal Component Analysis and Canonical Correlation Analysis on the NMR profiles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>These analyses revealed a significant predictive accuracy discriminating HI from GCP, and discriminating HI from GAgP patients (both 81%). In contrast, the GAgP and GCP saliva samples seem to belong to the same metabolic space (60% predictive accuracy). Significantly lower levels (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) of pyruvate, <jats:italic>N<\/jats:italic>\u2010acetyl groups and lactate and higher levels (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) of proline, phenylalanine, and tyrosine were found in GCP and GAgP patients compared with HI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Within the limitations of this study, CGP and GAgP metabolomic profiles were not unequivocally discriminated through a NMR\u2010based spectroscopic analysis of saliva.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.18-0097","type":"journal-article","created":{"date-parts":[[2018,6,7]],"date-time":"2018-06-07T06:30:40Z","timestamp":1528353040000},"page":"1452-1460","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Analysis of salivary phenotypes of generalized aggressive and chronic periodontitis through nuclear magnetic resonance\u2010based metabolomics"],"prefix":"10.1002","volume":"89","author":[{"given":"Federica","family":"Romano","sequence":"first","affiliation":[{"name":"Department of Surgical Sciences C.I.R. Dental School University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaia","family":"Meoni","sequence":"additional","affiliation":[{"name":"Magnetic Resonance Center (CERM) University of Florence  Sesto Fiorentino Italy"},{"name":"Department of Experimental and Clinical Medicine University of Florence  Sesto Fiorentino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Manavella","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences C.I.R. Dental School University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacomo","family":"Baima","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences C.I.R. Dental School University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Tenori","sequence":"additional","affiliation":[{"name":"Magnetic Resonance Center (CERM) University of Florence  Sesto Fiorentino Italy"},{"name":"Department of Experimental and Clinical Medicine University of Florence  Sesto Fiorentino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Cacciatore","sequence":"additional","affiliation":[{"name":"Department of Surgery &amp; Cancer Imperial College, London UK and International Centre for Genetic Engineering and Biotechnology Cancer Genomics Group  Cape Town South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Aimetti","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences C.I.R. 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