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Despite the advances in understanding the biology of PDL cells, there is a lack of information regarding the molecular signature of deciduous (DecPDL) and permanent (PermPDL) PDL tissues. Thus, the present study was designed to characterize the membrane proteome of DecPDL and PermPDL cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Primary PDL cells were obtained (n\u00a0=\u00a06) and a label\u2010free quantitative proteome of cell membrane\u2010enriched components was performed. Proteome findings were validated by quantitative polymerase chain reaction and Western blot assays in fresh human tissues (n\u00a0=\u00a08) and primary cell cultures (n\u00a0=\u00a06). In addition, confocal microscopy was used to verify the expression of target factors in the PDL cell cultures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Comparative gene ontology enrichment analysis evidenced that most stickling differences involved \u201cendomembrane system\u201d (PICALM, STX4, and LRP10), \u201chydrolase activity\u201d (NCSTN and XRCC6), \u201cprotein binding\u201d (PICALM, STX4, GPNMB, VASP, extended\u2010synaptotagmin 2 [ESYT2], and leucine\u2010rich repeat containing 15 [LRRC15]), and \u201cisomerase activity\u201d (FKBP8). Data are available via ProteomeXchange with identifier PXD010226. At the transcript level, high <jats:italic>PICALM<\/jats:italic> in DecPDL and <jats:italic>ESYT2<\/jats:italic> and <jats:italic>LRRC15<\/jats:italic> in PermPDL were confirmed in fresh PDL tissues. Furthermore, Western blot analysis confirmed increased levels of PICALM, LRRC15, and ESYT2 in cells and\/or fresh tissues, and confocal microscopy confirmed the trends for PICALM and LRRC15 expression in PDL cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We report the first comprehensive characterization of the membrane protein machinery of DecPDL and PermPDL cells, and together, we identified a distinct molecular signature for these cell populations, including unique proteins for DecPDL and PermPDL.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.18-0217","type":"journal-article","created":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T01:24:56Z","timestamp":1543541096000},"page":"775-787","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Membrane proteome characterization of periodontal ligament cell sets from deciduous and permanent teeth"],"prefix":"10.1002","volume":"90","author":[{"given":"Priscila A.","family":"Giovani","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry Piracicaba Dental School University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane R.","family":"Salmon","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Periodontics Division of Periodontics Piracicaba Dental School University of Campinas  Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciane","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Periodontics Division of Periodontics Piracicaba Dental School University of Campinas  Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana F. P.","family":"Leme","sequence":"additional","affiliation":[{"name":"Brazilian Biosciences National Laboratory LNBio CNPEM  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina M.","family":"Puppin\u2010Rontani","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Piracicaba Dental School University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana S.","family":"Mofatto","sequence":"additional","affiliation":[{"name":"Department of Genetics, Evolution and Bioagents Institute of Biology University of Campinas  Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Francisco H.","family":"Nociti","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Periodontics Division of Periodontics Piracicaba Dental School University of Campinas  Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila R.","family":"Kantovitz","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Piracicaba Dental School University of Campinas  Campinas S\u00e3o Paulo Brazil"},{"name":"Department of Dental Materials S\u00e3o Leopoldo Mandic Research Center  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(98)00054-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001940204"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2016.08.026"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.130.2.407"},{"key":"e_1_2_8_6_1","volume-title":"StemBook [Internet]","author":"Thesleff 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