{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:08:46Z","timestamp":1787756926977,"version":"build-2784847793"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,7]],"date-time":"2018-10-07T00:00:00Z","timestamp":1538870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005279","name":"Universidade Federal do Piau\u00ed","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005279","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Periodontitis not only causes injury to the periodontium, but also damages other tissues such as: articulate, renal, cardiac, and hepatic. The objective of this study was to investigate periodontitis induced alterations in liver function and structure using an experimental model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty female rats (<jats:italic>Rattus norvegicus<\/jats:italic>) were allocated into two groups: control and periodontitis. Gingival bleeding index and oxidative stress parameters and specific circulating biomarkers were measured. Immunohistochemistry was carried out using alkaline phosphatase (AlkP) staining of the liver. Hepatic tissues, cytokines, and lipid contents were measured. Histopathologic evaluation of the liver was carried out using light and electron microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Liver histopathologic and immunohistochemistry assessment showed increase in steatosis score, and presence of binucleate hepatocytes and positive cells for AlkP in periodontitis versus control group. Ultrastructural evaluation showed significant increase in size and number of lipid droplets (LD), distance between the cisterns of rough endoplasmic reticulum (RER), mitochondria size, foamy cytoplasm, and glycogen accumulation in the liver of the periodontitis group compared with the control group. In addition, plasma levels of AlkP, high\u2010density lipoprotein (HDL), triglycerides, and total cholesterol were also changed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Experimental periodontitis caused immunohistochemistry, histopathologic, ultrastructural, oxidative, and biochemical changes in the liver of rats.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.18-0226","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T04:03:06Z","timestamp":1537070586000},"page":"295-305","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Periodontitis causes abnormalities in the liver of rats"],"prefix":"10.1002","volume":"90","author":[{"given":"Any Carolina Cardoso Guimar\u00e3es","family":"Vasconcelos","sequence":"first","affiliation":[{"name":"Medicinal Plants Research Center (NPPM) Federal University of Piau\u00ed  Teresina PI Brazil"},{"name":"Medicine School Education Institute of Parnaiba Valley (IESVAP)  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Fernando Pereira","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Laboratory of Histological Analysis and Preparation (LAPHIS) Federal University of Piau\u00ed  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Rodolfo","family":"Pereira da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Histological Analysis and Preparation (LAPHIS) Federal University of Piau\u00ed  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Felipe","family":"de Carvalho Fran\u00e7a","sequence":"additional","affiliation":[{"name":"Laboratory of Histological Analysis and Preparation (LAPHIS) Federal University of Piau\u00ed  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Even Herlany Pereira","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Histological Analysis and Preparation (LAPHIS) Federal University of Piau\u00ed  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Di","family":"Lenardo","sequence":"additional","affiliation":[{"name":"Laboratory of Histological Analysis and Preparation (LAPHIS) Federal University of Piau\u00ed  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"dos Santos Pessoa","sequence":"additional","affiliation":[{"name":"Laboratory of Histological Analysis and Preparation (LAPHIS) Federal University of Piau\u00ed  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Duarte","family":"Novaes","sequence":"additional","affiliation":[{"name":"Piracicaba Dental School State University of Campinas \u2013 UNICAMP Department of Morphology Division of Histology  Piracicaba SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Luiz dos Reis Barbosa","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Physiopharmacology (LAFFEX) Federal University of Piau\u00ed  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arya","family":"Mani","sequence":"additional","affiliation":[{"name":"Yale Cardiovascular Research Center Department of Internal Medicine Yale University School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via Sammartino","family":"Mariano","sequence":"additional","affiliation":[{"name":"Piracicaba Dental School State University of Campinas \u2013 UNICAMP Department of Morphology Division of Histology  Piracicaba SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jand\u2010Venes Rolim","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Physiopharmacology (LAFFEX) Federal University of Piau\u00ed  Parna\u00edba PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alde\u00eddia Pereira","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Medicinal Plants Research Center (NPPM) Federal University of Piau\u00ed  Teresina PI Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,7]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"661","article-title":"The global burden of oral diseases and risks to oral health","volume":"83","author":"Petersen PE","year":"2005","journal-title":"Bull World Health Organ"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2015.140520"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1997.tb00760.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12628"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516634619"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"e0182284","DOI":"10.1371\/journal.pone.0182284","article-title":"Association between periodontitis and the risk of palindromic rheumatism: a nationwide, population\u2010based, case\u2010control study","volume":"8","author":"Lin CH","year":"2017","journal-title":"PLoS One"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12729"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12380"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.070056"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-12-16"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12459"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2016.160392"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v7.i6.846"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20734"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2008.10.009"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i43.9488"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200011163432007"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/979515"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01011-14"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-14260-9"},{"key":"e_1_2_8_22_1","first-page":"319","article-title":"A physiologic function for alkaline phosphatase: endotoxin detoxification","volume":"76","author":"Poelstra K","year":"1997","journal-title":"Lab Invest"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.08.002"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00235.2011"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27715"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i42.5280"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2014.10.633"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2009.06142.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/494578"},{"key":"e_1_2_8_30_1","first-page":"89","article-title":"Relationship between periodontitis and hepatic condition in Japanese women","volume":"8","author":"Saito T","year":"2006","journal-title":"J Int Acad Periodontol"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12800"},{"key":"e_1_2_8_32_1","first-page":"e269","article-title":"One or two ligatures inducing periodontitis are sufficient to cause fatty liver","volume":"23","author":"Pessoa LS","year":"2018","journal-title":"Med Oral Patol Oral Cir Bucal"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3109\/08923973.2012.707211"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2006.03.018"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20838"},{"key":"e_1_2_8_36_1","first-page":"549\u2010555","article-title":"Increased liver mast cell recruitment in patients with chronic C virus\u2010related hepatites and histologically documented steatosis","volume":"14","author":"Franceschini B","year":"2017","journal-title":"J Viral Hepat"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19960064"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2009.8"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-407-01571-5.50008-2"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90118-X"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(68)90092-4"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90342-1"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2015.03.002"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12448"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.5604\/16652681.1226818"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2012.128"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-013-9483-x"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.3897"},{"key":"e_1_2_8_50_1","first-page":"1641","article-title":"Cellular and molecular mechanisms of liver injury","volume":"6","author":"Malhi H","year":"2011","journal-title":"Gastroenterology"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2010.00292.x"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2FJPER.18-0226","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/JPER.18-0226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T08:59:38Z","timestamp":1760864378000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1002\/JPER.18-0226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,7]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/JPER.18-0226"],"URL":"https:\/\/doi.org\/10.1002\/jper.18-0226","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,7]]},"assertion":[{"value":"2018-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}