{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:34:31Z","timestamp":1762324471891,"version":"build-2065373602"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T00:00:00Z","timestamp":1557446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Though the use of salivary miRNAs as potential biomarkers has been reported in few diseases\/conditions such as rheumatoid arthritis and oral cancer, there are no reported studies on their utility in periodontal diagnostics. Thus, the aim of the present study was to profile salivary miRNAs and identify the most suitable salivary miRNA biomarker in chronic periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we have explored the potential application of next generation sequencing (NGS) technology for profiling miRNAs in two unstimulated saliva samples collected by passive drool method from a patient diagnosed with generalized chronic periodontitis and a healthy control. Subsequently, the validation of most highly expressed known miRNA in periodontitis was performed in saliva samples collected from an independent set of 16 chronic periodontitis patients and 16 periodontally healthy controls using quantitative real\u2010time PCR (qRT\u2010PCR). Target gene prediction and pathway mapping were performed using bioinformatic tools.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NGS analysis identified 40 upregulated and 40 downregulated known miRNAs in chronic periodontitis compared to healthy controls, of which miR\u2010143\u20103p was the most highly expressed miRNA in periodontitis (Read count \u2013 227630; fold change \u2013 5.82). Validation using qRT\u2010PCR showed significant upregulation of miR\u2010143\u20103p expression in the test group compared with controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). K\u2010RAS (V\u2010Ki\u2010ras2 Kirsten rat sarcoma viral oncogene) gene was predicted as the target gene for miR\u2010143\u20103p in humans. KEGG (Kyoto Encyclopedia of genes and genomes) pathway mapping revealed the involvement of K\u2010RAS in mitogen\u2010activated protein kinases (MAPK) pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The application of NGS for miRNA expression profiling can be considered a valuable tool in detection of novel biomarkers in periodontal diagnostics. Also, the results of the study points to the potential utility of miR143\u20103p as a novel salivary biomarker for chronic periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.18-0729","type":"journal-article","created":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T11:33:40Z","timestamp":1556192020000},"page":"1149-1159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Identification of a novel salivary biomarker miR\u2010143\u20103p for periodontal diagnosis: A proof of concept study"],"prefix":"10.1002","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1401-6604","authenticated-orcid":false,"given":"K.J.","family":"Nisha","sequence":"first","affiliation":[{"name":"Department of Periodontics Vydehi Institute of Dental Sciences and Research Centre  Bangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Presanthila","family":"Janam","sequence":"additional","affiliation":[{"name":"Department of Periodontics PMS College of Dental Science and Research  Thiruvananthapuram Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Harshakumar","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Government Dental College  Thiruvananthapuram Kerala 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