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The use of probiotics is widely extended in the case of gastrointestinal disorders; however, their use in microbial\u2010origin oral diseases is still preliminary.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used quantitative polymerase chain reaction to determine the levels of the oral bacterium <jats:italic>Streptococcus dentisani<\/jats:italic> 7746 in the tongue, saliva, supragingival, and subgingival plaque. We explore the potential benefits of this probiotic by measuring inhibition of the periodontal pathogens <jats:italic>Porphyromonas gingivalis<\/jats:italic> and <jats:italic>Fusobacterium nucleatum<\/jats:italic> growth and attachment to human gingival fibroblasts. In addition, its anti\u2010inflammatory activity against cytokines secretion induced by these pathogens was determined in an <jats:italic>in vitro<\/jats:italic> model by enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We report that <jats:italic>S. dentisani<\/jats:italic> is found at high levels in the gingival crevice. Data show a strong inhibitory action of <jats:italic>S. dentisani<\/jats:italic> supernatant against the periodontal pathogens in pure culture. <jats:italic>S. dentisani<\/jats:italic> attached to gingival cells <jats:italic>in vitro<\/jats:italic>, inhibiting periodontal pathogens by competition, adherence, and displacement mechanisms. Finally, in a simple <jats:italic>in vitro<\/jats:italic> model, the oral probiotic strongly increased the secretion of the anti\u2010inflammatory cytokine IL\u201010 after incubations with <jats:italic>P. gingivalis<\/jats:italic> and <jats:italic>F. nucleatum<\/jats:italic>, as well as significantly reduced the expression of interferon\u2010\u03b3 induced by <jats:italic>F. nucleatum<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Altogether, these results highlight the potential of <jats:italic>S. dentisani<\/jats:italic> as adjuvant therapy in the management of periodontal diseases, whose efficacy will need to be tested in clinical studies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.18-0751","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T03:44:30Z","timestamp":1558410270000},"page":"1346-1355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["In vitro beneficial effects of <i>Streptococcus dentisani<\/i> as potential oral probiotic for periodontal diseases"],"prefix":"10.1002","volume":"90","author":[{"given":"Adelaida","family":"Esteban\u2010Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Microbiology Institute of Food Science Research (CIAL) CSIC\u2010UAM  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria D.","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Department of Health and Genomics Center for Advanced Research in Public Health FISABIO Foundation  Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Zorraqu\u00edn\u2010Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology Institute of Food Science Research (CIAL) CSIC\u2010UAM  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arantxa","family":"L\u00f3pez\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Health and Genomics Center for Advanced Research in Public Health FISABIO Foundation  Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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