{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T01:51:41Z","timestamp":1792547501090,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T00:00:00Z","timestamp":1574208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102018R1A5A2023879"],"award-info":[{"award-number":["NRF\u20102018R1A5A2023879"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102019R1A2C4070083"],"award-info":[{"award-number":["NRF\u20102019R1A2C4070083"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study was performed to investigate the effects of cyclosporine A (CsA) on the osteogenic differentiation, osteoclastogenic\u2010supporting ability, and angiogenic potential of human periodontal ligament stem cells (hPDLSCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>hPDLSCs were isolated from the extracted teeth of orthodontic patients. Cell proliferation was assessed using 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102, 5\u2010diphenyltetrazolium bromide assay, and osteogenic differentiation was evaluated by alkaline phosphatase (ALP) and alizarin red (ARS) staining. Real\u2010time polymerase chain reaction (PCR) was used to quantify transcripts. Tartrate\u2010resistant acid phosphatase staining of bone marrow\u2010derived macrophages (BMMs) and tube formation assays on human umbilical vein endothelial cells (HUVECs) were performed after treating cells with the conditioned media from CsA\u2010exposed or non\u2010exposed hPDLSCs. Signaling pathways mediating the angiogenic activity were investigated using western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CsA suppressed the proliferation of hPDLSCs but enhanced osteogenic differentiation as determined by ALP and ARS staining and PCR of osteogenic transcripts. The expressions of osteoclastogenic transcripts in hPDLSCs and the differentiation of BMMs treated with conditioned medium from CsA\u2010exposed hPDLSCs were unaffected by CsA. However, the expressions of angiogenic transcripts and the transcripts known to support angiogenesis\u2014phosphorylation of extracellular signal p\u2010regulated kinase (ERK) and p38, and c\u2010fos\u2014were inhibited. Conditioned medium from CsA\u2010exposed hPDLSCs suppressed the tube forming abilities of HUVECs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CsA enhanced the osteogenic differentiation and reduced angiogenesis by blocking the ERK and p38\/c\u2010fos pathway in hPDLSCs. It is necessary to confirm whether this phenomenon is also observed in vivo in subsequent animal experiments.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.19-0168","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T02:08:11Z","timestamp":1572833291000},"page":"836-848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Assessing the effects of cyclosporine A on the osteoblastogenesis, osteoclastogenesis, and angiogenesis mediated by human periodontal ligament stem cells"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7553-6289","authenticated-orcid":false,"given":"Hyun\u2010Joo","family":"Kim","sequence":"first","affiliation":[{"name":"Dental Research Institute Pusan National University Dental Hospital  Yangsan Republic of Korea"},{"name":"Department of Periodontology and Institute of Translational Dental Science School of Dentistry Pusan 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