{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:34:07Z","timestamp":1780508047175,"version":"3.54.1"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T00:00:00Z","timestamp":1571875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hyperglycemic micro\u2010environment induced by diabetes could regulate the response of periodontal tissues to pathogenic microorganisms in which disruption of autophagy lysosomal pathway (ALP) may participate. This study aimed to explore the mechanisms underlying how high glucose (HG) regulates ALP in gingival epithelial cells (GECs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human gingival tissues in healthy group (C), periodontitis group (P), diabetes group (DM), and diabetes + periodontitis group (DP) were collected and were applied to observe the fusion of autophagy and lysosome. Diabetic mouse model with periodontitis was established and RNA\u2010seq was applied to investigate the expression of ALP\u2010associated genes in gingival epithelium. To explore the key role of ATPase transmembrane v0 domain, subunit c (ATP6V0C) in the disruption of ALP by HG, human gingival epithelial cells (HGECs) were cultured in 5.5\u00a0mM\/25\u00a0mM glucose medium for 48\u00a0hours and followed by <jats:italic>Porphyromonas gingivalis<\/jats:italic> stimulation for 0, 6, and 12\u00a0hours. HBLV\u2010h\u2010<jats:italic>ATP6V0C<\/jats:italic> was transfected in HGECs that were stimulated by 25\u00a0mM HG condition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunofluorescence double staining exhibited the disruption of ALP in human gingival epithelium in diabetes groups and HGECs under 25\u00a0mM glucose condition, accompanied with significantly downregulated lysosomal acidity. RNA\u2010seq of mouse gingival epithelium screened out <jats:italic>Atp6v0c<\/jats:italic>. Compared with HGECs in normal culture medium, ATP6V0C expression and LC3\u2010II\/I expression ratio were significantly downregulated, with an upregulated expression of P62, IL\u20101\u03b2 in HGECs under HG condition. Over\u2010expression of ATP6V0C rescued HG\u2010induced disruption of ALP in HBLV\u2010h\u2010<jats:italic>ATP6V0C<\/jats:italic> transfected HGECs, with significantly upregulation of LC3\u2010II\/I and downregulation of P62, IL\u20101\u03b2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>ATP6V0C mediates HG\u2010induced ALP disruption in HGECs, eventually exacerbates periodontal inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.19-0262","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T23:09:39Z","timestamp":1567206579000},"page":"705-714","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["High glucose disrupts autophagy lysosomal pathway in gingival epithelial cells via ATP6V0C"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9924-1055","authenticated-orcid":false,"given":"Xin","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Periodontology Guanghua School and Hospital of Stomatology Sun Yat\u2010Sen University  Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010Sen University North Campus  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhong","family":"Kuang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010Sen University North Campus  Guangzhou Guangdong China"},{"name":"Department of Operative Dentistry and Endodontics Guanghua School and Hospital of Stomatology Sun Yat\u2010Sen University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongshan","family":"Shen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010Sen University North Campus  Guangzhou Guangdong China"},{"name":"Department of Operative Dentistry and Endodontics Guanghua School and Hospital of Stomatology Sun Yat\u2010Sen University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Periodontology Guanghua School and Hospital of Stomatology Sun Yat\u2010Sen University  Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010Sen University North Campus  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengmei","family":"Lin","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010Sen University North Campus  Guangzhou Guangdong China"},{"name":"Department of Operative Dentistry and Endodontics Guanghua School and Hospital of Stomatology Sun Yat\u2010Sen University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc09-S062"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1996.1.1.821"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.16.1.329"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1349-0079(11)80006-X"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2011.08.022"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-157-4_4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1100356"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.02.013"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.10.492"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12141"},{"key":"e_1_2_8_12_1","first-page":"694","article-title":"Effects of bisphosphonates on Beclin1 and LC3\u01c1 induced by high\u2010glucose in rat bone marrow mesenchymal stem cells","volume":"49","author":"Liu Y","year":"2018","journal-title":"Sichuan Da Xue Xue Bao Yi Xue Ban"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00024"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.17-0733"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc14-S081"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81294-5"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2013.05.002"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710335105"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.060252"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.0022-0337.2002.66.4.tb03536.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.103"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20151938"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1239679"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2272"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-012110-142317"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.08.005"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2014.12.016"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/JPER.19-0262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/JPER.19-0262","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/JPER.19-0262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:31:45Z","timestamp":1760877105000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1002\/JPER.19-0262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,24]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1002\/JPER.19-0262"],"URL":"https:\/\/doi.org\/10.1002\/jper.19-0262","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,24]]},"assertion":[{"value":"2019-04-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}