{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:43:46Z","timestamp":1791045826371,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,9,22]],"date-time":"2019-09-22T00:00:00Z","timestamp":1569110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H04366"],"award-info":[{"award-number":["17H04366"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H17082"],"award-info":[{"award-number":["17H17082"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although numerous reports have demonstrated that the junctional epithelium (JE) is derived from the reduced enamel epithelium (REE), the fate of the REE\u2010derived JE remains controversial. The present study elucidated the fate of the REE\u2010derived JE and the cell dynamics of stem\/progenitor cells in the JE following tooth eruption.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mandibular first molar germs (embryonic days 15 to postnatal 1\u2010day\u2010old) were transplanted into the socket of 2\u2010week\u2010old mice after extraction of the upper first molars of B6 wild\u2010type (WT) and green fluorescent protein (GFP) transgenic mice. After analysis by \u00b5\u2010CT, paraffin sections were processed for immunohistochemistry for Nestin, Ki67 and GFP. We also performed chasing analysis using BrdU\u2010administered TetOP\u2010H2B\u2010GFP mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Amelogenesis progressed normally in the cervical areas, and the structure of the JE was like that in normal tooth development. The JE was GFP\u2010negative in transplantations using GFP transgenic mice as the host, and the oral epithelium (OE) showed a positive reaction. By contrast, the JE remained GFP\u2010positive throughout the experimental period in transplantations using GFP transgenic mice as the donor. Chasing analysis revealed that H2B\u2010GFP\u2010 and 5\u2010Bromo\u20102\u2032\u2010deoxyuridine (BrdU)\u2010labeled cells in the basal side of the JE translocated to oral side of the JE as the chasing time increased. Some label\u2010retaining cells remained at the supra\u2010basal cell layer in the JE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that REE\u2010derived cell niche in the JE is maintained for a long time following tooth eruption. Therefore, the JE may be available as the source of the odontogenic epithelium.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.19-0269","type":"journal-article","created":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T03:00:35Z","timestamp":1567998035000},"page":"819-827","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Reduced enamel epithelium\u2010derived cell niche in the junctional epithelium is maintained for a long time in mice"],"prefix":"10.1002","volume":"91","author":[{"given":"Miki","family":"Soda","sequence":"first","affiliation":[{"name":"Division of Pediatric Dentistry Department of Oral Health Science Niigata University Graduate School of Medical and Dental Sciences  Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kotaro","family":"Saito","sequence":"additional","affiliation":[{"name":"Division of Anatomy and Cell Biology of the Hard Tissue Department of Tissue 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