{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T21:50:42Z","timestamp":1772488242372,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T00:00:00Z","timestamp":1579478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004392","name":"Stiftelsen Patentmedelsfonden f\u00f6r Odontologisk Profylaxforskning","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004348","name":"Stockholms L\u00e4ns Landsting","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Colony\u2010stimulating factor 1 (CSF\u20101) and interleukin (IL)\u201034 are important for the functions of myeloid lineage cells and are involved in several chronic inflammatory conditions associated with tissue degeneration. The aim of this study is to evaluate the expression of CSF\u20101 and IL\u201034 in gingival tissue and gingival fibroblasts (GF) from patients with periodontitis and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Gingival biopsies were obtained from 19 periodontitis patients and 15 controls. Expression of CSF\u20101 and IL\u201034 in gingival tissue was assessed by western blot and localization by immunohistochemistry. Expression of <jats:italic>CSF1<\/jats:italic> and <jats:italic>IL34<\/jats:italic> mRNA in GF was analyzed by real\u2010time polymerase chain reaction and protein expression visualized by immunofluorescence stainings. CSF\u20101 and IL\u201034 secretion from GF was evaluated in response to tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), IL\u20101\u03b2, <jats:italic>Escherichia coli<\/jats:italic> lipopolysaccharide (Ec\u2010LPS) and <jats:italic>Porphyromonas gingivalis<\/jats:italic> lipopolysaccharide (Pg\u2010LPS) stimulation, using enzyme\u2010linked immunosorbent assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CSF\u20101 was increased in gingival tissue from periodontitis patients compared with controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) whereas IL\u201034 expression was similar. In GF from a non\u2010periodontitis donor, stimulation with either TNF\u2010\u03b1, IL\u20101\u03b2, Ec\u2010LPS, or Pg\u2010LPS, increased the secretion of CSF\u20101 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and Ec\u2010LPS stimulation increased IL\u201034 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). CSF\u20101 and IL\u201034 were expressed and secreted constitutively from GF, with comparable levels in GF from periodontitis patients and controls. Inflammatory stimuli increased the secretion of CSF\u20101 and IL\u201034 with comparable levels measured from GF from periodontitis patients and controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The expression of CSF\u20101 and IL\u201034 in gingival tissue and fibroblasts suggests involvement in myeloid cell functions during periodontal inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.19-0296","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T06:59:48Z","timestamp":1572850788000},"page":"828-835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Expression of colony\u2010stimulating factor 1 and interleukin\u201034 in gingival tissue and gingival fibroblasts from periodontitis patients and controls"],"prefix":"10.1002","volume":"91","author":[{"given":"Reuben","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Dental Medicine Division of Oral Diseases Karolinska Institutet  Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Zwicker","sequence":"additional","affiliation":[{"name":"Department of Dental Medicine Division of Oral Diseases Karolinska Institutet  Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Bureik","sequence":"additional","affiliation":[{"name":"Department of Dental Medicine Division of Oral Diseases Karolinska Institutet  Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunnar","family":"Johannsen","sequence":"additional","affiliation":[{"name":"Department of Dental Medicine Division of Oral Diseases Karolinska Institutet  Huddinge 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