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Yes\u2010associated protein (YAP), a mechanical stressor protein, may play an important role in this process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Western blot and quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma. Through local administration of <jats:italic>Porphyromonas gingivalis<\/jats:italic> and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models. Treatment with or without XAV939, to inhibit YAP activation, was performed in these models. Micro\u2010computed tomography, immunofluorescence (IF), and qRT\u2010PCR were used to explore the YAP pathway in periodontitis with occlusal trauma. Cyclic stress and lipopolysaccharide (LPS) stimuli were applied to the L929 mouse fibroblast cell line with or without XAV939. Western blot, IF, and qRT\u2010PCR were used to verify the <jats:italic>in vivo<\/jats:italic> results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma. In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N\u2010terminal kinases (JNK) related pro\u2010inflammatory pathway up\u2010regulation. L929 cell cyclic stress and LPS stimulation results confirmed the <jats:italic>in vivo<\/jats:italic> results. Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro\u2010inflammatory pathway factor expression <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Occlusal trauma can activate YAP nuclear transfer, resulting in the up\u2010regulation of the JNK pro\u2010inflammatory pathway. This can be inhibited by the XAV939 YAP inhibitor.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.19-0338","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T02:57:20Z","timestamp":1603421840000},"page":"1036-1048","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Inhibition of yes\u2010associated protein dephosphorylation prevents aggravated periodontitis with occlusal trauma"],"prefix":"10.1002","volume":"92","author":[{"given":"Wei","family":"Wei","sequence":"first","affiliation":[{"name":"The State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Department of Prosthodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of stomatology the First 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