{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:27:55Z","timestamp":1780568875543,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,4,18]],"date-time":"2020-04-18T00:00:00Z","timestamp":1587168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Occlusal trauma is an important local contributing factor aggravating periodontal pocket and alveolar bone absorption in periodontal diseases. Our previous studies have found that occlusal trauma inhibited osteogenic differentiation through nuclear factor (NF)\u2010\u03baB signaling. To further investigate the underlying mechanism, the aim of this study was to explore the role of long chain non\u2010coding differentiation antagonizing non\u2010protein coding RNA (Dancr) in the inhibitory effect of traumatic stress on osteoblast differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We took the MC3T3\u2010E1 cells as object in vitro research and stimulated cells with simple stress load, Dancr\u2010siRNA + stress load, Dancr overexpression\u2010plasmid + stress load. Quantitative real\u2010time polymerase chain reaction was used to detect the RNA expression levels of Dancr, alkaline phosphatase (Alp) and Runt\u2010related transcription factor 2 (Runx2). The protein expressions of Alp and Runx2 were tested by Western blot and the activity of Alp was qualitatively demonstrated by Alp staining. In addition, Western blot was performed to investigate the role of Dancr in affecting NF\u2010\u03baB signaling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Traumatic compressive stress inhibited the expressions of Alp, Runx2, andDancr in MC3T3\u2010E1 cells. Stress\u2010induced inhibition of osteoblast differentiation was promoted after silencing Dancr. Overexpression of Dancr could alleviate the inhibitory effect of traumatic force on osteoblast differentiation to some extent. Furthermore, NF\u2010\u03baB signaling was activated after silencing Dancr, and the activated effect of traumatic force on NF\u2010\u03baB signaling could be alleviated through overexpression of Dancr to some extent.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Traumatic compressive stress can indirectly activate the NF\u2010\u03baB signaling through downregulation of Dancr, thereby inhibiting osteogenic differentiation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.19-0648","type":"journal-article","created":{"date-parts":[[2020,3,11]],"date-time":"2020-03-11T14:53:30Z","timestamp":1583938410000},"page":"1532-1540","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Traumatic compressive stress inhibits osteoblast differentiation through long chain non\u2010coding RNA Dancr"],"prefix":"10.1002","volume":"91","author":[{"given":"Qian","family":"Lu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Sichuan University  Chengdu Sichuan China"},{"name":"Department of Cariology and Endodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weizhe","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Sichuan University  Chengdu Sichuan China"},{"name":"Department of Cariology and Endodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linyi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Sichuan University  Chengdu Sichuan China"},{"name":"Department of Cariology and Endodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuedong","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Sichuan University  Chengdu Sichuan China"},{"name":"Department of Cariology and Endodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Sichuan University  Chengdu Sichuan China"},{"name":"Department of Cariology and Endodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongzhe","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Sichuan University  Chengdu Sichuan China"},{"name":"Department of Cariology and Endodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Sichuan University  Chengdu Sichuan China"},{"name":"Department of Cariology and Endodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingming","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases Sichuan University  Chengdu Sichuan China"},{"name":"Department of Cariology and Endodontics, West China Hospital of Stomatology Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12091"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2006.0050"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00514.x"},{"key":"e_1_2_8_5_1","first-page":"1","article-title":"Risk for periodontal disease in a Swedish adult population. 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