{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:22:58Z","timestamp":1790886178701,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,9,20]],"date-time":"2020-09-20T00:00:00Z","timestamp":1600560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Persistent host immune responses initiated by oral bacteria protect host against infection but may also elicit the process of sustained periodontal inflammation and subsequent alveolar bone loss. Interleukin\u201010 (IL\u201010), an anti\u2010inflammatory cytokine, can downregulate pro\u2010inflammatory cytokine and inhibit neutrophil migration in inflammation. IL\u201010\u2010expressing regulatory B cells (B10) is termed by negatively regulating immune response through IL\u201010 and are mainly restricted in CD19<jats:sup>+<\/jats:sup>CD1d<jats:sup>hi<\/jats:sup>CD5<jats:sup>+<\/jats:sup>B cells in mice. Our current study was aimed to explore the effect of locally transferred CD19<jats:sup>+<\/jats:sup>CD1d<jats:sup>hi<\/jats:sup>CD5<jats:sup>+<\/jats:sup>B cells on inflammation and alveolar bone loss in an experimental periodontitis mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ligation plus <jats:italic>P<\/jats:italic>. <jats:italic>gingivalis<\/jats:italic> (Pg) infection was used to induce periodontitis in a mouse model. CD19<jats:sup>+<\/jats:sup>CD1d<jats:sup>hi<\/jats:sup>CD5<jats:sup>+<\/jats:sup> B cells were sorted by flow cytometry and transferred into the gingivae immediately on the fifth day after ligation. All the mice were sacrificed on day 14 after ligation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>H&amp;E staining showed that inflammatory cell infiltration was significantly reduced by the CD19<jats:sup>+<\/jats:sup>CD1d<jats:sup>hi<\/jats:sup>CD5<jats:sup>+<\/jats:sup> B cells. Toluidine blue staining showed that the CD19<jats:sup>+<\/jats:sup>CD1d<jats:sup>hi<\/jats:sup>CD5<jats:sup>+<\/jats:sup> B cells alleviated alveolar bone loss in the ligature\/Pg\u2010induced periodontitis in mice. Immunohistochemical staining showed Receptor Activator of NF\u2010KappaB Ligand (RANKL), Interleukin\u20101\u03b2(IL\u20101\u03b2) and Interleukin\u201017 (IL\u201017) were decreased after the CD19<jats:sup>+<\/jats:sup>CD1d<jats:sup>hi<\/jats:sup>CD5<jats:sup>+<\/jats:sup>B cell transfer. Immunofluorescent staining showed that IL\u201010 was increased while the number of Ly6G<jats:sup>+<\/jats:sup> neutrophil and its RANKL production were decreased in gingival tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicated that locally transferred CD19<jats:sup>+<\/jats:sup>CD1d<jats:sup>hi<\/jats:sup>CD5<jats:sup>+<\/jats:sup>B cells may alleviate alveolar bone loss through inhibiting pro\u2010inflammatory cytokine expression and RANKL\u2010expressing neutrophils in the periodontitis mouse model.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.20-0074","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T14:13:11Z","timestamp":1599055991000},"page":"907-920","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Local promotion of B10 function alleviates experimental periodontitis bone loss through antagonizing RANKL\u2010expressing neutrophils"],"prefix":"10.1002","volume":"92","author":[{"given":"Guoqin","family":"Cao","sequence":"first","affiliation":[{"name":"Department of stomatology The Fourth Hospital of Harbin Medical University  Harbin China"},{"name":"Department of stomatology The First People hospital of Jingzhou  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of stomatology The Fourth Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Song","sequence":"additional","affiliation":[{"name":"Department of stomatology The Fourth Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of stomatology The Fourth Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Immunology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhe","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases The Forsyth Institute  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of stomatology The Fourth Hospital of Harbin Medical University  Harbin China"},{"name":"Department of stomatology Beijing Tongren hospital of Capital Medical University  Beijing 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