{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:32:15Z","timestamp":1787761935269,"version":"build-2784847793"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T00:00:00Z","timestamp":1605225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To explore the diagnostic usefulness of extracellular vesicles (EVs), and their subpopulations (micro\u2010vesicles and exosomes), and microRNAs (miRNA\u201021\u20103p, miRNA\u2010150\u20105p, and miRNA\u201026a\u20105p) in peri\u2010implant crevicular fluid (PICF) of subjects with healthy, peri\u2010implant mucositis and peri\u2010implantitis implants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 54 patients were enrolled into healthy, peri\u2010implant mucositis, and peri\u2010implantitis groups. PICF samples were collected, EVs subpopulations (MVs and Exo) were isolated and characterized by nanoparticle tracking analysis and transmission electron microscopy. The expression of miRNA\u201021\u20103p, miRNA\u2010150\u20105p and miRNA\u201026a\u20105p was quantified by qRT\u2010PCR. Logistic regression models and accuracy performance tests were estimated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PICF samples show the presence of EVs delimited by a bi\u2010layered membrane, in accordance with the morphology and size\u00a0(&lt;\u00a0200\u00a0nm). The concentration of PICF\u2010EVs, micro\u2010vesicles and exosomes was significantly increased in peri\u2010implantitis implants compared to healthy implants (<jats:italic>P\u00a0=\u00a00.023<\/jats:italic>, <jats:italic>P\u00a0=\u00a00.002<\/jats:italic>, <jats:italic>P\u00a0=\u00a00.036<\/jats:italic>, respectively). miRNA\u201021\u20103p and miRNA\u2010150\u20105p expression were significantly downregulated in patients with peri\u2010implantitis in comparison with peri\u2010implant mucositis sites (<jats:italic>P\u00a0=\u00a00.011, P\u00a0=\u00a00.020, respectively<\/jats:italic>). The reduced expression of miRNA\u201021\u20103p and miRNA\u2010150\u20105p was associated with peri\u2010implantitis diagnosis (OR:0.23, CI 0.08\u20100.66, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.007 and OR:0.07, CI 0.01\u20100.78, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.031, respectively). The model which included the miRNA\u201021\u20103p and miRNA\u2010150\u20105p expression had a sensitivity of 93.3%, a specificity of 76.5%, a positive predictive value of 77.8%, and a negative predictive value of 92.9%. The positive and negative likelihood ratios were 3.97 and 0.09, respectively. The area under the receiver operating characteristics curve for the model was 0.84.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>An increase concentration of EVs with a downregulation expression of miRNA\u201021\u20103p and miRNA\u2010150\u20105p could be related with the peri\u2010implantitis development.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.20-0372","type":"journal-article","created":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T11:58:39Z","timestamp":1605268719000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Diagnostic potential of peri\u2010implant crevicular fluid microRNA\u201021\u20103p and microRNA\u2010150\u20105p and extracellular vesicles in peri\u2010implant diseases"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0791-7746","authenticated-orcid":false,"given":"Alejandra","family":"Chaparro","sequence":"first","affiliation":[{"name":"Department of Periodontology, Centro de Investigaci\u00f3n e Innovaci\u00f3n Biom\u00e9dica (CIIB), Faculty of Dentistry Universidad de los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6298-9756","authenticated-orcid":false,"given":"Pablo","family":"Atria","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Centro de Investigaci\u00f3n e Innovaci\u00f3n Biom\u00e9dica (CIIB), Faculty of Dentistry Universidad de los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ornella","family":"Realini","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Centro de Investigaci\u00f3n e Innovaci\u00f3n Biom\u00e9dica (CIIB), Faculty of Dentistry Universidad de los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara J.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Centro de Investigaci\u00f3n e Innovaci\u00f3n Biom\u00e9dica (CIIB), Faculty of Medicine Universidad de los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Betancur","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry Universidad de Concepci\u00f3n  Concepci\u00f3n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Acu\u00f1a\u2010Gallardo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Centro de Investigaci\u00f3n e Innovaci\u00f3n Biom\u00e9dica (CIIB), Faculty of Medicine Universidad de los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Department of Statistics and Epidemiology, Faculty of Dentistry Universidad de los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Bendek","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Centro de Investigaci\u00f3n e Innovaci\u00f3n Biom\u00e9dica (CIIB), Faculty of Dentistry Universidad de los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Pascual","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry Universitat Internacional de Catalunya  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Nart","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School 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