{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:01:46Z","timestamp":1787508106252,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:00:00Z","timestamp":1610668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Several potential benefits have been attributed to the platelet\u2010rich fibrin (PRF), including enhanced tissue healing properties. In this study, we hypothesized that the application of PRF as an adjunct to conventional scaling and root planing (ScRp) would enhance the outcomes of non\u2010surgical periodontal therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The present study was a split\u2010mouth randomized controlled clinical trial design in 24 deep periodontal pockets in 12 patients with periodontitis. The pockets were randomly assigned as test or control. The test group received PRF as an adjunct to ScRp, whereas the control group received ScRp only. We measured periodontal clinical parameters at baseline, 3, and 6 months after the treatments. To study the initial healing in response to treatment, transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) and collagen\u20101 (Col\u20101) in gingival crevicular fluid (GCF) were measured using enzyme\u2010linked immunosorbent assay at baseline, third, seventh, and 14th days.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The test group showed a significantly greater pocket reduction, higher clinical attachment gain, and less gingival recession than the control group at 3 and 6 months. The test Col\u20101 levels (1.27 \u00b1 1.05, 1.35 \u00b1 0.76, 0.97 \u00b1 0.53\u00a0ng\/site) and TGF\u2010\u03b2 levels (11.93 \u00b1 2.68, 12.54 \u00b1 3.66, 17.19 \u00b1 11.66 pg\/site) were higher than the control Col\u20101 levels (0.76 \u00b1 0.20, 0.84 \u00b1 0.24, 0.57 \u00b1 0.19\u00a0ng\/site) and TGF\u2010\u03b2 levels (6.34 \u00b1 1.67, 6.35 \u00b1 3.44, 7.51 \u00b1 2.85 pg\/site) at all measurement days respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Non\u2010surgical application of the PRF as an adjunct to conventional ScRp may effectively improve the periodontal clinical parameters via increasing expression of the GCF TGF\u2010\u03b2 and Col\u20101 levels.<\/jats:p>\n                  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