{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T10:16:24Z","timestamp":1768990584898,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cellular cementum, a mineralized tissue covering apical tooth roots, grows by apposition to maintain the tooth in its occlusal position. We hypothesized that resident cementocytes would show morphological changes in response to cementum apposition, possibly implicating a role in cementum biology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mandibular first molars were induced to super\u2010erupt (EIA) by extraction of maxillary molars, promoting rapid new cementum formation. Tissue and cell responses were analyzed at 6 and\/or 21 days post\u2010procedure (dpp).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High\u2010resolution micro\u2010computed tomography (micro\u2010CT) and confocal laser scanning microscopy showed increased cellular cementum by 21 dpp. Transmission electron microscopy (TEM) revealed that cementocytes under EIA were 50% larger than control cells, supported by larger pore sizes detected by micro\u2010CT. Cementocytes under EIA displayed ultrastructural changes consistent with increased activity, including increased cytoplasm and nuclear size. We applied EIA to <jats:italic>Hyp<\/jats:italic> mutant mice, where cementocytes have perilacunar hypomineralization defects, to test cell and tissue responses in an altered mechanoresponsive milieu. <jats:italic>Hyp<\/jats:italic> and WT molars displayed similar super\u2010eruption, with <jats:italic>Hyp<\/jats:italic> molars exhibiting 28% increased cellular cementum area versus 22% in WT mice at 21 dpp. Compared to control, <jats:italic>Hyp<\/jats:italic> cementocytes featured well\u2010defined, disperse euchromatin and a thick layer of peripherally condensed heterochromatin in nuclei, indicating cellular activity. Immunohistochemistry (IHC) for cementum markers revealed intense dentin matrix protein\u20101 expression and abnormal osteopontin deposition in <jats:italic>Hyp<\/jats:italic> mice. Both WT and <jats:italic>Hyp<\/jats:italic> cementocytes expressed gap junction protein, connexin 43.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides new insights into the EIA model and cementocyte activity in association with new cementum formation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.21-0119","type":"journal-article","created":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T09:33:38Z","timestamp":1621330418000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cementocyte alterations associated with experimentally induced cellular cementum apposition in <i>Hyp<\/i> mice"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0324-3863","authenticated-orcid":false,"given":"Elis J.","family":"Lira dos Santos","sequence":"first","affiliation":[{"name":"Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School State University of Campinas  S\u00e3o Paulo Brazil"},{"name":"Division of Biosciences, College of Dentistry The Ohio State University  Columbus OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiane R.","family":"Salmon","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School State University of Campinas  S\u00e3o Paulo Brazil"},{"name":"Faculty of Dentistry N. 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