{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:57:59Z","timestamp":1773003479376,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T00:00:00Z","timestamp":1631145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Excessive inflammation in the periodontal tissue after tooth replantation can lead to inflammatory root resorption and interrupt periodontal tissue regeneration. We tested the hypothesis that nuclear factor\u2010\u03baB decoy oligodeoxynucleotide\u2010loaded poly lactic\u2010co\u2010glycolic acid nanospheres (NF\u2010PLGA) inhibit excessive inflammation and promote healing of periodontal tissue after replantation in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The upper right incisors of rats were extracted, immersed in different specific solutions, and replanted. The rats were euthanized at 7, 14, and 28 days after replantation. Morphological evaluation with micro\u2010CT and histological assessment with hematoxylin and eosin and tartrate\u2010resistant acid phosphatase (TRAP) staining was performed. Additionally, we examined the expression of interleukin (IL)\u20101\u03b2, IL\u20106, transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21), and fibroblast growth factor\u20102 (FGF\u20102) in the periodontal ligament (PDL) by performing immunohistological assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The NF\u2010PLGA group showed significantly greater dental root thickness than the other experimental groups. Root resorption was not observed after the application of NF\u2010PLGA on day 7. The application of NF\u2010PLGA also resulted in a significantly lower number of TRAP\u2010positive osteoclasts on days 7 and 14 after replantation. Significantly lower expression of IL\u20101\u03b2 and IL\u20106 and higher expression of TGF\u2010\u03b21 and FGF\u20102 were observed under the application of NF\u2010PLGA in the PDL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>NF\u2010PLGA promoted the healing process by inhibiting the initial excessive inflammatory response in the PDL, preventing root resorption, and promoting periodontal tissue regeneration. The findings also suggested that the PLGA nanospheres\u2010mediated transfection of the decoy oligodeoxynucleotides can be useful for the clinical application of replanted tooth root surfaces.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.21-0134","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T08:43:40Z","timestamp":1627461820000},"page":"458-470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Nuclear factor\u2010kappa B decoy oligodeoxynucleotide\u2010loaded poly lactic\u2010co\u2010glycolic acid nanospheres promote periodontal tissue healing after tooth replantation in rats"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5881-577X","authenticated-orcid":false,"given":"Kai","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kasumi","family":"Hatano\u2010Sato","sequence":"additional","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7031-2697","authenticated-orcid":false,"given":"Narubhorn","family":"Ongprakobkul","sequence":"additional","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"},{"name":"Department of Orthodontics, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Hosomichi","sequence":"additional","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Risa","family":"Usumi\u2010Fujita","sequence":"additional","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sawa","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"},{"name":"Department of Pediatrics The University of Texas Medical School at Houston  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Orthodontic Science, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.2000.tb00234.x"},{"key":"e_1_2_8_3_1","first-page":"329","article-title":"Etiology and pathogenesis of traumatic dental injuries. 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