{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:17:27Z","timestamp":1784290647498,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T00:00:00Z","timestamp":1636675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cell\u2013cell communication is an essential process to respond to biological stimuli and sustain the micro environmental homeostasis of human periodontal ligament stem cells (hPDLSCs). Connective tissue growth factor (CTGF), a critical secreted matrix protein, exhibits significant tasks in regulating the cell\u2010cell and cell\u2010matrix interactions. This study aimed to explore the relationship between CTGF and cell communication and the underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>qRT\u2010PCR was used to detect CCN family, connexin, and pannexin family expression in hPDLSCs. Stimulation with CTGF, cell migration assay was performed to examine the wound repair. The scrape loading\/dye transfer assay was employed to access lucifer Yellow molecules transfer efficiency mediated by cell\u2010cell communication. Connexin43 (Cx43), Pannexin1 (Panx1), MAPK, and the PI3K\/Akt signaling pathway proteins were examined via Western blotting. Immunofluorescence was applied to visualize the localization of specific proteins within cells. Corresponding pathway inhibitors were applied to hPDLSCs to detect Cx43, Panx1 expression, and intercellular communication induced by CTGF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our result showed that CTGF was the second most expressed CCN family member in hPDLSCs. Cx43, and Panx1 were the most widely expressed gap junction hemichannels in hPDLSCs. CTGF enhanced hPDLSCs migration in a dose\u2010dependent manner. CTGF promoted cell\u2010cell communication by up\u2010regulating Cx43 and Panx1. CTGF induced Akt, JNK, and p38 phosphorylation and subcellular relocation. Inhibiting corresponding pathways reduced Cx43 expression, thereby weakening CTGF\u2010induced cell\u2010cell communication. However, the Panx1 expression in CTGF\u2010treated hPDLSCs mainly depended on PI3K\/Akt signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We provided novel evidence that CTGF promoted cell\u2010cell communication in hPDLSCs through MAPK and PI3K pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.21-0339","type":"journal-article","created":{"date-parts":[[2021,9,17]],"date-time":"2021-09-17T04:52:32Z","timestamp":1631854352000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Connective tissue growth factor promotes cell\u2010to\u2010cell communication in human periodontal ligament stem cells via MAPK and PI3K pathway"],"prefix":"10.1002","volume":"93","author":[{"given":"Zuping","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"State Key Laboratory of 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