{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:13:00Z","timestamp":1790273580428,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T00:00:00Z","timestamp":1643241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>L\u2010arginine (L\u2010arg) can reduce apoptosis in a variety of cells. Cementoblast apoptosis is related to root resorption during orthodontic treatment. In the present study, we aimed to study the regulatory effect and potential mechanism of L\u2010arg on cementoblast apoptosis and root resorption.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The apoptosis\u2010related mRNA and protein expression of murine cementoblast (OCCM\u201030) was assessed after L\u2010arg treatment. To investigate the role of Sirtuin 1 (Sirt1) and autophagy in L\u2010arg resistance to cementoblast apoptosis and root absorption, resveratrol, and EX527 were used to activate or inhibit Sirt1, and chloroquine (CQ) was used to inhibit autophagy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vitro, L\u2010arg inhibited hypoxia\u2010induced apoptosis in OCCM\u201030. Further, L\u2010arg increased Sirt1 expression whereas Sirt1 suppression by EX527 reversed the inhibitory effect of L\u2010arg on cell apoptosis. Sirt1 activator resveratrol increased the ratio of microtubule\u2011associated protein light chain 3 (LC3) II\/I and decreased the expression of SQSTM1\/p62 (p62), suggesting autophagy activation. Autophagy enhancement could reduce apoptosis. Caspase\u20103 and Bax expression was decreased, and Bcl\u20102 expression was increased. When autophagy was inhibited by CQ, the positive effects of Sirt1 were attenuated. In vivo, L\u2010arg application reduced root resorption in rats, as demonstrated by decreased root absorption volume. Similarly, L\u2010arg upregulated Sirt1, which activated autophagy in the root resorption model, and less root resorption was observed in the Sirt1 activation group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>L\u2010arg reduced cementoblast apoptosis in hypoxia and reduced root resorption induced by loading force in rats, which may be partly mediated by Sirt1\u2010enhanced autophagy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.21-0473","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T00:04:50Z","timestamp":1640563490000},"page":"1961-1973","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["L\u2010arginine protects cementoblasts against hypoxia\u2010induced apoptosis through Sirt1\u2010enhanced autophagy"],"prefix":"10.1002","volume":"93","author":[{"given":"Lei","family":"Xu","sequence":"first","affiliation":[{"name":"College of Stomatology 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