{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T23:24:34Z","timestamp":1781565874135,"version":"3.54.5"},"reference-count":50,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study aimed to evaluate the levels of total matrix metalloproteinase\u20108 (MMP\u20108), macrophage\u2010activating factors (MAF), macrophage inflammatory protein (MIP)\u20101\u03b1, macrophage colony\u2010stimulating factor (M\u2010CSF), interleukin (IL)\u201034 in saliva, and serum of periodontally healthy, periodontitis Stage III Grade B (P\u2010III\u2010B) and Grade C (P\u2010III\u2010C) participants and to compare the changes after non\u2010surgical periodontal treatment (NSPT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of non\u2010smoker and systemically healthy 65 participants, 20 periodontally healthy, 20 P\u2010III\u2010B, and 25 P\u2010III\u2010C were recruited for the study. The periodontal parameters were recorded, saliva and serum samples were obtained from all participants at baseline. In periodontitis groups, the periodontal parameters were reevaluated, and the samples were recollected at 1 and 3 months following the NSPT. MMP\u20108, MAF, MIP\u20101\u03b1, M\u2010CSF, and IL\u201034 levels were measured by ELISA. Receiver operating characteristics curve was performed for estimating the area under the curve (AUC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All periodontal parameters were improved in periodontitis groups after NSPT (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). Among tested molecules, salivary MMP\u20108 and MAF were higher in both periodontitis groups compared to healthy controls (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) at baseline and significantly decreased after NSPT (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) to healthy levels or below. Salivary MMP\u20108 had the highest diagnostic ability both for P\u2010III\u2010B (AUC:0.78 sensitivity: 80%; specificity: 80%) and P\u2010III\u2010C (AUC:0.88 sensitivity: 88%; specificity: 80%). Moreover, after adjusting for age, periodontitis groups were associated with salivary MMP\u20108 and MAF levels (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present study showed that high salivary MMP\u20108 and MAF levels were associated with non\u2010smoker, systemically healthy P\u2010III\u2010B and P\u2010III\u2010C. Moreover, NSPT was remarkably reduced their levels.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.21-0536","type":"journal-article","created":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T08:24:41Z","timestamp":1642580681000},"page":"1191-1205","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effect of non\u2010surgical periodontal treatment on salivary and serum biomarkers in Stage III Grade B and C periodontitis"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0890-9695","authenticated-orcid":false,"given":"Nimet G\u00fcl","family":"G\u00f6rg\u00fcl\u00fc","sequence":"first","affiliation":[{"name":"Department of Periodontology, Institute of Health Science Marmara University  Istanbul 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