{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T23:52:22Z","timestamp":1773100342469,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T00:00:00Z","timestamp":1672099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study evaluated the effects of systemic administration of <jats:italic>Bifidobacterium animalis subsp. lactis<\/jats:italic> HN019 (<jats:italic>B. lactis<\/jats:italic> HN019) on experimental periodontitis (EP) in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010two rats were allocated to groups C (control), C\u2010HN019 (probiotic), EP (EP only), and EP\u2010HN019 (EP+probiotic). From day 0, the animals of C\u2010HN019 and EP\u2010HN019 groups received <jats:italic>B. lactis<\/jats:italic> HN019 (1\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup> CFU\/ml) daily. On the 14th day, the animals of EP and EP\u2010HN019 groups received silk ligature around mandibular molars. Animals were euthanized on the 28th day. Samples of oral biofilm, gingival tissues, blood serum, and mandible were obtained for microtomographic, histomorphometric, microbiological, and immunological analyses. Data were statistically analyzed (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Group EP\u2010HN019 presented significantly less alveolar bone loss when compared with Group EP in histomorphometric and microtomographic analyses. In gingival tissue and serum, Group EP\u2010HN019 presented lower proinflammatory\/anti\u2010inflammatory cytokines ratios than Group EP. Group EP\u2010HN019 showed higher expression of beta\u2010defensins and less TRAP\u2010positive cells than Group EP. Group EP presented higher gene expression of <jats:italic>Ifng<\/jats:italic> and lower gene expression of <jats:italic>Foxp3<\/jats:italic> when compared with Group EP\u2010HN019 in gingival tissue. In oral biofilm, EP\u2010HN019 group presented a lower percentage of species similar to <jats:italic>Fusobacterium periodonticum<\/jats:italic> and a higher percentage of species similar to <jats:italic>Actinomyces gereneseriae, Actinomyces israelli<\/jats:italic>, and <jats:italic>Streptococcus gordonii<\/jats:italic> when compared with Group EP. There was a significant increase of <jats:italic>B. lactis<\/jats:italic> HN019 after administration of probiotic therapy in oral biofilm of Group EP\u2010HN019.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The consumption of <jats:italic>B. lactis<\/jats:italic> HN019 promotes a protective effect against alveolar bone loss by modifying local and systemic microbiological and immunoinflammatory parameters.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.21-0555","type":"journal-article","created":{"date-parts":[[2022,6,12]],"date-time":"2022-06-12T08:13:56Z","timestamp":1655021636000},"page":"217-229","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Probiotic consumption can modify the resilience of periodontal tissues in rats under experimental periodontitis"],"prefix":"10.1002","volume":"94","author":[{"given":"Luiz Fernando Ferreira","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo \u2013 USP  Ribeir\u00e3o Preto \/ SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7583-7046","authenticated-orcid":false,"given":"Pedro Henrique Felix","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo \u2013 USP  Ribeir\u00e3o Preto \/ SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4867-7294","authenticated-orcid":false,"given":"Sergio Luiz","family":"Salvador","sequence":"additional","affiliation":[{"name":"Department of Clinical Analyses School of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo \u2010 USP  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