{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:45:11Z","timestamp":1787067911613,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,4,9]],"date-time":"2022-04-09T00:00:00Z","timestamp":1649462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Saliva, salivary glands, gingival crevicular fluid, and supragingival biofilms may harbor SARS\u2010CoV\u20102 RNA. This observational study aimed to investigate the presence and load of SARS\u2010CoV\u20102 RNA in supragingival, and subgingival biofilms obtained from intensive care unit (ICU) patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A convenience sample, composed of 52 COVID\u201019+ participants (48.6 \u00b1 14.8 years, 26.9% females), were evaluated for pre\u2010existing comorbidities, number of teeth, and periodontal data [visible plaque (VPI), bleeding on probing (BOP), periodontal probing depth (PPD), and attachment loss (AL)]. Supragingival and subgingival samples (SubDeep: four sites with the deepest PPD; SubRemain: remaining shallower sites) were analyzed by RT\u2010qPCR with corresponding cycle quantification (Cq). Statistical analyses considered the individual (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a05%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Twenty\u2010six participants tested positive for dental biofilms (Biofilm+) with 96.2% of them being positive for subgingival samples. Pre\u2010existing comorbidities, number of teeth examined, VPI, PPD, AL, and BOP were similar between Biofilm+ and Biofilm\u2010. SubDeep PPD (3.72 \u00b1 0.86), AL (4.34 \u00b1 1.33), and % of BOP (66.0 \u00b1 31.1) values were significantly greater compared to SubRemain values (2.84 \u00b1 0.48, 3.37 \u00b1 0.34, and 20.4 \u00b1 24.1, respectively). Biofilm+ Cqs showed no association with the periodontal condition. Cqs from Nasopharynx\/Oropharynx (Naso\/Oro; n\u00a0=\u00a036) were similar between Biofilm+ and Biofilm\u2010 participants. Length of time since ICU intake, last Naso\/Oro RT\u2010qPCR readings, onset of COVID\u201019 symptoms, and biofilm samplings were greater for Biofilm\u2010.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ICU patients harbored SARS\u2010CoV\u20102 RNA in supragingival and subgingival biofilms, irrespective of the periodontal condition, and systemic viral load. The high number of positive patients highlights the need to better understand this habit to provide adequate oral care.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jper.21-0623","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T12:53:45Z","timestamp":1646312025000},"page":"1476-1485","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["SARS\u2010CoV\u20102 RNA in dental biofilms: Supragingival and subgingival findings from inpatients in a COVID\u201019 intensive care unit"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0102-9043","authenticated-orcid":false,"given":"Sabrina C.","family":"Gomes","sequence":"first","affiliation":[{"name":"Department of Conservative Dentistry Dental School Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Rio Grande do Sul Brazil"},{"name":"Hospital de Cl\u00ednicas de Porto Alegre (HCPA)  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane G.","family":"da Fonseca","sequence":"additional","affiliation":[{"name":"Periodontology Resident Dental School UFRGS  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00edsa M.","family":"Miller","sequence":"additional","affiliation":[{"name":"Graduate Student Dental School UFRGS  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciane","family":"Manenti","sequence":"additional","affiliation":[{"name":"Graduate Student Dental School UFRGS  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Daniela M.","family":"Angst","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry Dental School Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Rio Grande do Sul Brazil"},{"name":"Hospital de Cl\u00ednicas de Porto Alegre (HCPA)  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo L.","family":"Lamers","sequence":"additional","affiliation":[{"name":"Department of Morphological Sciences Dental School UFRGS  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilma S.","family":"Brum","sequence":"additional","affiliation":[{"name":"Department of Physiology Basic Health Science Institute UFRGS  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana N.","family":"Nunes","sequence":"additional","affiliation":[{"name":"Mathematics and Statistics Institute UFRGS  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/eci.13484"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2020.12.007"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001\u2010020\u201000475\u20106"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25805"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaerosci.2020.105617"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11070993"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010021\u201000644\u2010x"},{"key":"e_1_2_11_9_1","unstructured":"World Health Organization (WHO).Update on Omicron.2021. 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