{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:41:20Z","timestamp":1760920880108,"version":"build-2065373602"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T00:00:00Z","timestamp":1659571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The aim of this study was to evaluate oral bacteria\u2010 and interleukin (IL)\u20101\u03b2\u2010induced protein and mRNA expression profiles of monocyte chemoattractant protein\u20101\u2010induced protein (MCPIP)\u20101 and mucosa\u2010associated lymphoid tissue lymphoma translocation protein (MALT)\u20101 in human gingival keratinocyte monolayers and organotypic oral mucosal models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human gingival keratinocyte (HMK) monolayers were incubated with <jats:italic>Porphyromonas gingivalis<\/jats:italic>, <jats:italic>Fusobacterium nucleatum<\/jats:italic>, <jats:italic>P. gingivalis<\/jats:italic> lipopolysaccharide (LPS) and IL\u20101\u03b2. The protein levels of MCPIP\u20101 and MALT\u20101 were examined by immunoblots and mRNA levels by qPCR. MCPIP\u20101 and MALT\u20101 protein expression levels were also analyzed immunohistochemically using an organotypic oral mucosal model. One\u2010way analysis of variance followed by Tukey correction was used in statistical analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In keratinocyte monolayers, MCPIP\u20101 protein expression was suppressed by <jats:italic>F. nucleatum<\/jats:italic> and MALT\u20101 protein expression was suppressed by <jats:italic>F. nucleatum<\/jats:italic>, <jats:italic>P. gingivalis<\/jats:italic> LPS and IL\u20101\u03b2. <jats:italic>P. gingivalis<\/jats:italic> seemed to degrade MCPIP\u20101 and MALT\u20101 at all tested time points and degradation was inhibited when <jats:italic>P. gingivalis<\/jats:italic> was heat\u2010killed. MCPIP\u20101 mRNA levels were increased by <jats:italic>P. gingivalis<\/jats:italic>, <jats:italic>F. nucleatum<\/jats:italic>, and IL\u20101\u03b2, however, no changes were observed in MALT\u20101 mRNA levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Gingival keratinocyte MCPIP\u20101 and MALT\u20101 mRNA and protein expression responses are regulated by infection and inflammatory mediators. These findings suggest that periodontitis\u2010associated bacteria\u2010induced modifications in MCPIP\u20101 and MALT\u20101 responses can be a part of periodontal disease pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.22-0093","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T03:55:25Z","timestamp":1655438125000},"page":"130-140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Regulation of gingival keratinocyte monocyte chemoattractant protein\u20101\u2010induced protein (MCPIP)\u20101 and mucosa\u2010associated lymphoid tissue lymphoma translocation protein (MALT)\u20101 expressions by periodontal bacteria, lipopolysaccharide, and interleukin\u20101\u03b2"],"prefix":"10.1002","volume":"94","author":[{"given":"Yigit","family":"Firatli","sequence":"first","affiliation":[{"name":"Department of Periodontology, Institute of Dentistry University of Turku  Turku Finland"},{"name":"Department of Periodontology, Faculty of Dentistry Istanbul University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erhan","family":"Firatli","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry Istanbul University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vuokko","family":"Loimaranta","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Institute of Dentistry University of Turku  Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"Elmanfi","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Institute of Dentistry University of Turku  Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1225-5751","authenticated-orcid":false,"given":"Ulvi K.","family":"G\u00fcrsoy","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Institute of Dentistry University of Turku  Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8081135"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201344202"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40496-020-00249-3"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12594"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/000163501750266710"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00191.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdsr.2017.06.001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004952"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2020.1795584"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00502-21"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.09.012"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20092641"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.06.029"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.627922"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.727861"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sigtrans.2017.66"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.034"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4564"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2002.73.1.86"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2010.090664"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110535"},{"key":"e_1_2_9_23_1","first-page":"10","article-title":"Regulation of hBD\u20102, hBD\u20103, hCAP18\/LL37, and proinflammatory cytokine secretion by human milk oligosaccharides in an organotypic oral mucosal model","volume":"11","author":"Gursoy UK","year":"2021","journal-title":"Pathogens"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.07452.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000339826"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01505.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2012.01780.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753425910379840"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12094"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21197183"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.2478\/prilozi-2020-0026"},{"key":"e_1_2_9_32_1","first-page":"1681972","article-title":"Fusobacterium nucleatum facilitates apoptosis, ROS generation, and inflammatory cytokine production by activating AKT\/MAPK and NF\u2010\u03baB signaling pathways in human gingival fibroblasts","volume":"13","author":"Kang W","year":"2019","journal-title":"Oxid Med Cell Longev"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-4-9-117"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2015.12.027"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2001031"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2018.05.009"},{"key":"e_1_2_9_37_1","first-page":"10(5)","article-title":"MALT1 protease activity is required for innate and adaptive immune responses","volume":"12","author":"Yu JW","year":"2015","journal-title":"PLoS One"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni0308-231"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2021.03.053"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2540"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3VMA0118-019R"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.10.1413"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.029132-0"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/JPER.22-0093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/JPER.22-0093","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/JPER.22-0093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:31:45Z","timestamp":1760880705000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1002\/JPER.22-0093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,4]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/JPER.22-0093"],"URL":"https:\/\/doi.org\/10.1002\/jper.22-0093","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2022,8,4]]},"assertion":[{"value":"2022-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}