{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T20:04:39Z","timestamp":1789416279750,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although periodontal ligament\u2010derived mesenchymal stem cells (PDL\u2010MSCs) have been recognized as the best MSC choice for periodontal regeneration, using PDL\u2010MSCs requires tooth extraction for cell isolation. The supracrestal gingival (SG) connective tissue is a part of the gingiva which is located close to the PDL. SG\u2010MSCs might have similar characteristic to the PDL\u2010MSCs and serve as a good MSC candidate for periodontal regeneration. This study aimed to investigate and compare the characteristics of human MSCs isolated from SG tissue (hSG\u2010MSCs), marginal gingival tissue (hMG\u2010MSCs), and PDL (hPDL\u2010MSCs) in terms of MSCs properties and differential gene expression profile.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human periodontal tissue from five healthy subjects, including SG, MG, and PDL, was harvested. The primary cells of the hSG\u2010MSCs, hMG\u2010MSCs, and hPDL\u2010MSCs were isolated and expanded to assess MSCs markers by flow cytometry, colony\u2010forming ability, differentiation potential, RNA sequencing, and real\u2010time polymerase chain reaction (PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the three cell types, the hSG\u2010MSCs demonstrated the highest colony\u2010forming ability. The number of alizarin red S positive colonies produced by the hSG\u2010MSCs was higher than the hMG\u2010MSCs but lower than the hPDL\u2010MSCs. RNA sequencing revealed that the hSG\u2010MSCs had a more similar gene expression profile to the hPDL\u2010MSCs than the hMG\u2010MSCs. Pathway enrichment analysis found no significant differences in the differentially expressed genes between the hSG\u2010MSCs and hPDL\u2010MSCs; however, there were significantly enriched pathways between the hPDL\u2010MSCs and hMG\u2010MSCs for the extracellular matrix (ECM) organization and ECM\u2010receptor interaction pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrated a close relationship between hSG\u2010MSCs and hPDL\u2010MSCs. hSG\u2010MSCs could be a potential MSC source for periodontal tissue engineering.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.22-0306","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T07:28:30Z","timestamp":1665473310000},"page":"439-450","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Characteristics of mesenchymal stem cells from supracrestal gingival connective tissue"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6272-8169","authenticated-orcid":false,"given":"Supreda S.","family":"Srithanyarat","sequence":"first","affiliation":[{"name":"Department of Periodontology Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Periodontal Disease and Dental Implant Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Genomics and Precision Dentistry Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manutsanun","family":"Choosiri","sequence":"additional","affiliation":[{"name":"Department of Periodontology Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noppadol","family":"Sa\u2010Ard\u2010Iam","sequence":"additional","affiliation":[{"name":"Center of Excellence in Periodontal Disease and Dental Implant Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pijitra","family":"Petcharat","sequence":"additional","affiliation":[{"name":"Oral Biology Research Center Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanaphum","family":"Osathanon","sequence":"additional","affiliation":[{"name":"Dental Stem Cell Biology Research Unit and Department of Anatomy Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13536"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010018\u20101093\u20109"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.08.121"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12086"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1909"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpor.2012.06.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.05.025"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2010.090566"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902318"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000360276"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0796"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22405"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.01.126"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902318"},{"key":"e_1_2_9_16_1","first-page":"19","volume-title":"Newman and Carranza's Clinical Periodontology","author":"Fiorellini JP","year":"2019"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102405"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12940"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1967.38.2.112"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.01.031"},{"key":"e_1_2_9_21_1","unstructured":"AndrewsS.FastQC: a quality control tool for high throughput sequence data.2018. 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