{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T21:45:08Z","timestamp":1774907108771,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,4,25]],"date-time":"2023-04-25T00:00:00Z","timestamp":1682380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic periodontitis (CP), the most prevalent dysbiotic bacteria\u2010driven chronic inflammatory disease, is an underestimated global health problem in itself, and due to a causative relationship with other disorders such as cardiovascular diseases or Alzheimer disease. The CP pathogenesis is primarily driven by <jats:italic>Porphyromonas gingivalis<\/jats:italic> in humans, and <jats:italic>Porphyromonas gulae<\/jats:italic> in dogs. These microorganisms initiate a pathogenic shift in the composition of the tooth\u2010surface microflora. Our objective was to evaluate antimicrobial effects of bestatin, a potential CP drug candidate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated bestatin bacteriostatic efficiency against periodontopathogens in planktonic cultures via microplate assay, and mono\u2010 and multispecies oral biofilm models. Neutrophil bactericidal activities, such as phagocytosis, were investigated in vitro using granulocytes isolated from the peripheral blood. The therapeutic efficacy and the immunomodulatory function of bestatin was assessed in a murine model of CP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bestatin exhibited bacteriostatic activity against both <jats:italic>P. gingivalis<\/jats:italic> and <jats:italic>P. gulae<\/jats:italic>, and controlled the formation and species composition of the biofilm. We demonstrated that bestatin promotes the phagocytosis of periodontopathogens by neutrophils. Finally, we found that providing bestatin in the animal feed prevented alveolar bone resorption.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We show that in a murine model of CP bestatin not only shifted the biofilm species composition from pathogenic to a commensal one, but also promoted bacteria clearance by immune cells and alleviated inflammation. Taken together, these results suggest that bestatin is a promising drug choice for the treatment and\/or prevention of periodontitis and clinical trials are required to fully evaluate its potency.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.22-0614","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T07:12:27Z","timestamp":1680765147000},"page":"1338-1350","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bestatin as a treatment modality in experimental periodontitis"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8299-7603","authenticated-orcid":false,"given":"Marta","family":"Kaminska","sequence":"first","affiliation":[{"name":"Broegelmann Research Laboratory Department of Clinical Science University of Bergen  Bergen 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