{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:26:33Z","timestamp":1789194393739,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:00:00Z","timestamp":1672963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Clinical experience indicates that wounds in alveolar bone and periodontal tissue heal faster and more efficiently in the maxilla compared with the mandible. Since stem cells are known to have a decisive influence on wound healing and tissue regeneration, the aim of this study was to determine whether differences in proliferation and differentiation of periodontal ligament stem cells (PDLSC) from upper (u\u2010PDLSC) and lower jaw (l\u2010PDLSC) contribute to the enhanced wound healing in the maxilla.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>u\u2010PDLSC and l\u2010PDLSC from the same donor were harvested from the periodontal ligament of extracted human maxillary and mandibular third molars. Cell differentiation potential was assessed by analyzing stem cell markers, proliferation rate, and multilineage differentiation among each other and bone marrow\u2010derived mesenchymal stem cells (MSC). Successful differentiation of PDLSC and MSC toward osteoblasts, adipocytes, and chondrocytes was analyzed via reverse transcriptase\u2010quantitative polymerase chain reaction and histochemical staining (Alizarin Red, Oil Red O, Toluidine Blue).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>u\u2010PDLSC and l\u2010PDLSC expressed the MSC\u2010markers CD73<jats:sup>+<\/jats:sup>, CD90<jats:sup>+<\/jats:sup>, and CD105<jats:sup>+<\/jats:sup> and lacked expression of CD34<jats:sup>\u2212<\/jats:sup> and CD45<jats:sup>\u2212<\/jats:sup>. Proliferation was significantly higher in u\u2010PDLSC than in l\u2010PDLSC, regardless of the culture conditions. Osteogenic (ALP, RunX2, and osteocalcin) and chondrogenic (SOX9 and ACAN) related gene expression as well as staining intensities were significantly higher in u\u2010PDLSC than in l\u2010PDLSC. No difference in adipogenic differentiation was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>u\u2010PDLSC showed a significantly higher proliferative and differentiation potential than l\u2010PDLSC, offering a possible cell\u2010based explanation for the differences in periodontal wound healing efficacy between maxilla and mandible.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.22-0706","type":"journal-article","created":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T11:28:11Z","timestamp":1671708491000},"page":"882-895","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Comparative analysis of proliferative and multilineage differentiation potential of human periodontal ligament stem cells from maxillary and mandibular molars"],"prefix":"10.1002","volume":"94","author":[{"given":"Sinan","family":"Mert","sequence":"first","affiliation":[{"name":"Helmholtz Institute for Biomedical Engineering, BioInterface Group RWTH Aachen University Hospital  Aachen Germany"},{"name":"Institute of Pathology RWTH Aachen University Hospital  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Malyaran","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Biomedical Engineering, BioInterface Group RWTH Aachen University Hospital  Aachen Germany"},{"name":"Institute of Pathology RWTH Aachen University Hospital  Aachen Germany"},{"name":"Interdisciplinary Center for Clinical Research (IZKF) RWTH Aachen University Hospital  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogerio B.","family":"Craveiro","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Dental Clinic RWTH Aachen University Hospital  Aachen 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