{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:49Z","timestamp":1786978129997,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In periodontitis, the equilibrium between bone formation and resorption skews in favor of bone loss. Periodontal ligament\u2010associated protein\u20101 (PLAP\u20101) and sclerostin play a significant role in the suppression of bone formation. Tumor necrosis factor\u2010alpha (TNF\u2010\u03b1) is a central proinflammatory cytokine related to periodontal bone loss. This study aims to assess gingival crevicular fluid (GCF) PLAP\u20101, sclerostin, and TNF\u2010\u03b1 levels in individuals with periodontal disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Seventy\u2010one individuals diagnosed with generalized stage III grade C periodontitis (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a023), gingivitis (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a024), and periodontal health (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a024) were included in the study. Full\u2010mouth clinical periodontal measurements were performed. PLAP\u20101, sclerostin, and TNF\u2010\u03b1 total amounts in GCF were quantified by ELISA. Nonparametric methods were used for the data analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Periodontitis group exhibited significantly higher GCF PLAP\u20101, sclerostin and TNF\u2010\u03b1 levels compared with gingivitis and periodontally healthy groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). GCF PLAP\u20101 and TNF\u2010\u03b1 levels of gingivitis group were higher than healthy controls (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) whereas GCF sclerostin levels were similar in two groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&gt;\u00a00.05). Significant positive correlations were found between GCF PLAP\u20101, sclerostin and TNF\u2010\u03b1 levels and all clinical parameters (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>To our knowledge, this is the first study showing GCF PLAP\u20101 levels in periodontal health and disease. Increased GCF PLAP\u20101 and sclerostin levels and their correlations with TNF\u2010\u03b1 in periodontitis imply that those molecules might be involved in the pathogenesis of periodontal disease. Further studies in larger mixed cohorts are needed to enlighten the possible role of PLAP\u20101 and sclerostin in periodontal bone loss.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jper.22-0750","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T03:01:00Z","timestamp":1680490860000},"page":"1166-1175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Gingival crevicular fluid periodontal ligament\u2010associated protein\u20101, sclerostin, and tumor necrosis factor\u2010alpha levels in periodontitis"],"prefix":"10.1002","volume":"94","author":[{"given":"Berkay","family":"G\u00fcr","sequence":"first","affiliation":[{"name":"Department of Periodontology Faculty of Dentistry Ayd\u0131n Adnan Menderes University  Ayd\u0131n 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