{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:02Z","timestamp":1786978082077,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Clinical probing is commonly recommended to evaluate peri\u2010implant conditions. In a situation of peri\u2010implant mucositis or peri\u2010implantitis, the peri\u2010implant seal healing from the disruption of soft tissue caused by probing has not yet been studied. This study aimed to investigate soft tissue healing after standardized clinical probing around osseointegrated implants with peri\u2010implant mucositis in a dog model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Three transmucosal implants in each hemi\u2010mandible of six dogs randomly assigned to the peri\u2010implant healthy group or peri\u2010implant mucositis group were probed randomly in the mesial or distal site as probing groups (PH or PM), the cross\u2010sectional opposite sites as unprobed control groups. Histomorphometric measurements of implant shoulder (IS)\u2013most coronal level of alveolar bone contact to the implant surface (BCI), apical termination of the junctional epithelium (aJE)\u2013BCI, mucosal margin (MM)\u2013BCI, and MM\u2013aJE were performed at 1\u00a0day, 1\u00a0week, and 2\u00a0weeks after probing. Apoptosis, proliferation, proinflammatory cytokines, and matrix metalloproteinases (MMPs) of peri\u2010implant soft tissue were estimated by immunofluorescent analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the PM group, apical migration of junctional epithelium was revealed by significantly decreased aJE\u2013BCI from 1\u00a0day to 2\u00a0weeks in comparison to unprobed sites (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), while no significant differences were found in the PH group. Immunofluorescent analysis showed higher levels of interleukin\u20101\u03b2 (IL\u20101\u03b2), IL\u20106, tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), MMP\u20101, and MMP\u20108, together with exaggerated apoptosis and proliferation of peri\u2010implant soft tissue in the PM group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Within the limitations, standardized clinical probing might lead to apical migration of the junctional epithelium in a situation of peri\u2010implant mucositis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jper.23-0167","type":"journal-article","created":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T10:45:18Z","timestamp":1690627518000},"page":"233-243","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Influences of standardized clinical probing on peri\u2010implant soft tissue seal in a situation of peri\u2010implant mucositis: A histomorphometric study in dogs"],"prefix":"10.1002","volume":"95","author":[{"given":"Rong","family":"Cai","sequence":"first","affiliation":[{"name":"State Key Laboratory of Military Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; Shaanxi Key Laboratory of Stomatology Department of Oral Implants School of Stomatology The Fourth Military Medical University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3267-2218","authenticated-orcid":false,"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; Shaanxi Key Laboratory of Stomatology Department of Oral Implants School of Stomatology The Fourth Military Medical University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinge","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; Shaanxi Key Laboratory of Stomatology Department of Oral Implants School of Stomatology The Fourth Military Medical University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbo","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology &amp; 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