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However, expression profiles of these factors in bone cells under diabetes mellitus (DM) and estrogen\u2010deficient conditions are poorly understood. This study investigated the immunoexpression of ANXA1 and its formyl peptide receptor 2 (FPR2), as well as NLRP3 inflammasome mediators, during remodeling of the alveolar process in diabetic and estrogen\u2010deficient rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty adult female Wistar rats were divided into four groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a05): Sham\u2010operated (SHAM) and ovariectomized (OVX) rats received a vehicle solution, and SHAM and OVX rats were intraperitoneally administered 60\u00a0mg\/kg\/body weight (BW) of streptozotocin (STZ) to induce DM (SHAM\u2010Di and OVX\u2010Di groups). After 7\u00a0weeks, the rats were euthanized and their maxillae were fixed in phosphate\u2010buffered 4% formaldehyde and embedded in paraffin. Sections were stained with hematoxylin\/eosin (H&amp;E) and picrosirius red or subjected to immunohistochemical detection of ANXA1, FPR2, NLRP3, interleukin\u20101\u03b2 (IL\u20101\u03b2), and cyclooxygenase\u20102 (COX2).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Estrogen deficiency and DM were associated with deleterious effects in bone tissue, as evidenced by a lower number of osteocytes and higher number of empty lacunae in the SHAM\u2010Di and OVX\u2010Di groups compared to the nondiabetic groups. Both diabetic groups showed a smaller vascular area and weaker collagen fiber birefringence intensity in alveolar bone tissue. A significantly higher number of ANXA1\/FPR2\u2010positive osteoblasts, osteocytes, and osteoclasts was accompanied by a significantly higher number of these cells immunolabeled for COX2, NLRP3, and IL\u20101\u03b2 in the diabetic and OVX groups, especially in both estrogen\u2010deficient and diabetic rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These results indicate a possible role for the ANXA1\/FPR2 pathway as a fine\u2010tuning\/anti\u2010inflammatory regulator to counterbalance exacerbated COX2\/NLRP3\/IL\u20101\u03b2 activation in bone cells during bone remodeling under estrogen deficiency and DM.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jper.23-0530","type":"journal-article","created":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T13:24:07Z","timestamp":1700659447000},"page":"749-763","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Possible role of annexin A1\/FPR2 pathway in COX2\/NLRP3 inflammasome regulation in alveolar bone cells of estrogen\u2010deficient female rats with diabetes mellitus"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6583-1329","authenticated-orcid":false,"given":"Gisela Rodrigues Da Silva","family":"Sasso","sequence":"first","affiliation":[{"name":"Department of Morphology and Genetics Laboratory of Histology and Structural Biology Federal University of S\u00e3o Paulo \u2013 Paulista School of Medicine (UNIFESP \u2013 EPM)  S\u00e3o Paulo SP 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