{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T14:19:56Z","timestamp":1784729996686,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:00:00Z","timestamp":1718841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Bacterial\u2010induced inflammation instigates the destruction of hard and soft tissues surrounding teeth in periodontitis. In severe cases, the increased number and activity of osteoclasts induces the resorption of alveolar bones, ultimately leading to tooth loss. Because of their diverse chemical structures and bioactivities, natural compounds are often suggested to treat a wide variety of diseases, including inflammatory disorders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the present study, we demonstrated an inhibitory effect of gossypetin, a hexahydroxy flavone, on osteoclast differentiation and bone resorption using in vitro culture of osteoclasts from mouse bone marrow macrophage (BMM) precursors and in vivo model of ligature\u2010induced periodontitis in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Gossypetin significantly reduced the differentiation of osteoclasts from mouse BMM precursors in the presence of the receptor activator of nuclear factor \u03baB ligand (RANKL). In vitro, gossypetin inhibited critical signaling events downstream of RANKL including the auto\u2010amplification of nuclear factor of activated T\u2010cells, cytoplasmic 1, Ca<jats:sup>2+<\/jats:sup> oscillations, and the generation of reactive oxygen species. In a mouse ligature\u2010induced periodontitis model, the administration of gossypetin significantly reduced osteoclastogenesis and alveolar bone resorption. Furthermore, gossypetin prevented the ligature\u2010induced increase in macrophages and T cells and reduced the production of tumor necrosis factor\u2010\u03b1 and interleukin\u20106.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Taken together, these results show anti\u2010osteoclastogenic and anti\u2010inflammatory effects of gossypetin, suggesting the potential use of this natural compound in periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.23-0541","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T01:33:16Z","timestamp":1718933596000},"page":"164-175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Flavonoid gossypetin protects alveolar bone and limits inflammation in ligature\u2010induced periodontitis in mice"],"prefix":"10.1002","volume":"96","author":[{"given":"Jiwon","family":"Seok","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Dentistry Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myoung Ok","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, College of Ecology and Environmental Science Kyungpook National University  Sangju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung\u2010Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010Medical Analysis Korea Polytechnic College  Chungnam South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka\u2010Young","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry Kyungpook National University  Daegu South 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