{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T21:47:45Z","timestamp":1789076865171,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Gingivitis is the most common form of periodontal disease among children and adolescents and is associated with disrupted host\u2013microbiome homeostasis. Family is an important factor influencing the prevalence of gingivitis. In the present study, we investigated the salivary microbiome, oral hygiene habits, and the salivary level of myeloid\u2010related protein (MRP)\u20108\/14 in children aged 7\u201312 years with gingivitis, periodontally healthy children, and their mothers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study included 24 children with gingivitis (including four sibling pairs) and 22 periodontally healthy children (including two sibling pairs) and their mothers. The whole saliva was collected, DNA was extracted, the variable V3\u2013V4 region of the eubacterial 16S ribosomal RNA gene was amplified, and sample library preparation was performed according to the Illumina protocol. The salivary levels of MRP\u20108\/14 were analyzed by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Alpha diversity of the salivary microbiome was considerably higher in gingivitis children and mothers of gingivitis children compared to healthy children and their mothers, respectively. Significant differences in beta diversity between healthy and gingivitis children, healthy children and their mothers, and gingivitis children and their mothers were detected. Overall, the number of common core amplicon sequence variants between children and their own mothers was significantly higher than between children and other mothers. The salivary MRP\u20108\/14 levels in children with gingivitis were significantly higher compared to healthy children; a similar tendency was also mentioned for mothers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study underlines the importance of family as an essential factor influencing oral health.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.23-0632","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T13:40:13Z","timestamp":1714657213000},"page":"1035-1047","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Salivary microbiome and MRP\u20108\/14 levels in children with gingivitis, healthy children, and their mothers"],"prefix":"10.1002","volume":"95","author":[{"given":"Alice","family":"Blufstein","sequence":"first","affiliation":[{"name":"Competence Center for Periodontal Research University Clinic of Dentistry Medical University of Vienna  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