{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:03:46Z","timestamp":1791644626580,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004368","name":"Colgate-Palmolive Company","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004368","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The aim of this study is to evaluate the immune regulation and tissue remodeling responses during experimental gingivitis (EG) and naturally occurring gingivitis (NG) to provide a comprehensive analysis of host responses. Gingival crevicular fluid (GCF) was obtained from 2 human studies conducted in university settings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The EG study enrolling 26 volunteers provided controls for the baseline (Day 0) from healthy disease\u2010free participants, while Day 21 (the end of EG induction of the same group) was used to represent EG. Twenty\u2010six NG participants age\u2010matched with those of the EG group were recruited. GCF samples were analyzed for 39 mediators of inflammatory\/immune responses and tissue remodeling using commercially available bead\u2010based multiplex immunoassays. The differences in GI and mediator expression among groups were determined at a 95% confidence level (<jats:italic>p<\/jats:italic> \u2264 0.05) by a 2\u2010way analysis of variance (ANOVA) with a post\u2010hoc Tukey's test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our findings showed that EG had a greater gingival index than NG and was healthy (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01 of all comparisons). Furthermore, EG showed significantly higher levels of MPO (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001), CCL3 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), and IL\u20101B (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) than NG. In contrast, NG had increased levels of MIF (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), Fractalkine (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001), angiogenin (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), C3a (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001), BMP\u20102 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001), OPN (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), RANKL (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001), and MMP\u201013 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) than EG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Consistent with the findings from chronic (NG) versus acute (EG) inflammatory lesions, these data reveal that NG displays greater immune regulation, angiogenesis, and bone remodeling compared to EG.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.23-0692","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T06:10:04Z","timestamp":1714975804000},"page":"1139-1149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Inflammation and tissue remodeling mediator expression during gingivitis: A comparison between experimental, naturally occurring gingivitis, and periodontal health"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0439-5578","authenticated-orcid":false,"given":"Yung\u2010Ting","family":"Hsu","sequence":"first","affiliation":[{"name":"Department of Periodontics University of Washington School of Dentistry  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Periodontics University of Washington School of Dentistry  Seattle Washington USA"},{"name":"College of Osteopathic Medicine Pacific Northwest University of Health Sciences  Yakima Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7307-4102","authenticated-orcid":false,"given":"Diane","family":"Daubert","sequence":"additional","affiliation":[{"name":"Department of Periodontics University of Washington School of Dentistry  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Roberts","sequence":"additional","affiliation":[{"name":"Department of Periodontics University of Washington School of Dentistry  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Periodontics University of Washington School of Dentistry  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsh M.","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Department of Periodontics University of Washington School of Dentistry  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Gomez","sequence":"additional","affiliation":[{"name":"Department of Periodontics University of Washington School of Dentistry  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rich P.","family":"Darveau","sequence":"additional","affiliation":[{"name":"Department of Periodontics University of Washington School of Dentistry  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12146"},{"key":"e_1_2_9_3_1","first-page":"235","article-title":"Pathogenesis of inflammatory periodontal disease. 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