{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T19:48:19Z","timestamp":1783367299911,"version":"3.54.6"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R03DE028632"],"award-info":[{"award-number":["R03DE028632"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE032334"],"award-info":[{"award-number":["R01DE032334"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cellular cementum (CC) includes cementocytes, cells suspected to regulate CC formation or resorption as osteocytes do in bone. Sclerostin (SOST) is a secreted negative regulator of Wnt\/\u03b2\u2010catenin signaling expressed by osteocytes and\u00a0cementocytes. Osteocyte SOST expression reduces bone formation. We investigated the functional importance of SOST in CC compared with alveolar bone (AB) using a <jats:italic>Sost<\/jats:italic> knockout (<jats:italic>Sost<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>) mouse model to better understand the role of cementocytes in CC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mandibles and femurs of <jats:italic>Sost<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> and wild\u2010type (WT) mice were analyzed at 42 and 120 days postnatal (dpn). Maxillary first molars were bilaterally extracted at 42 dpn and both AB healing (maxillary molar sockets) and CC apposition (mandibular first molars) were examined at 21 days post\u2010procedure. Analyses included micro\u2010computed tomography, histology, and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Femur cortical and trabecular bone and mandibular bone volumes were similarly increased in <jats:italic>Sost<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> versus WT mice at 42 and\/or 120 dpn. In contrast to previous reports, CC was not increased by <jats:italic>Sost<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> at either age. We conducted challenge experiments on AB and CC to explore tissue\u2010specific responses. Post\u2010extraction AB healing was improved by <jats:italic>Sost<\/jats:italic> deletion. In contrast, experimentally\u2010induced apposition in molars failed to stimulate increased CC formation in <jats:italic>Sost<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> versus WT mice. Wnt pathway markers AXIN2 and DKK1, which were increased in <jats:italic>Sost<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> versus WT AB osteocytes, were unchanged in cementocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data indicate CC is less responsive than AB to SOST deletion. Within the study limitations, these results do not support cementocytes as critical for directing increased CC formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Plain Language Summary<\/jats:title><jats:p>Sclerostin is a protein known to inhibit bone formation, and removing sclerostin leads to more bone formation. Cementum is the thin layer that covers the surface of the tooth's root. Previous studies suggest that inhibiting sclerostin can similarly increase the amount of cementum. We wanted to compare the response of cementum and bone when sclerostin is absent to understand similarities and differences between these two tissues. In this study, we removed the <jats:italic>Sost<\/jats:italic> gene (the gene which produces sclerostin) in mice. We found that mice without sclerostin have more bone in their legs and jaws. Moreover, mice without sclerostin also healed better after tooth removal compared with normal mice. Surprisingly, unlike previous studies, we found that the amount of cementum was not different in mice without sclerostin compared with normal mice. Additionally, we challenged the cementum by taking out the opposing tooth to cause the first mandibular molar to move up by building more cementum. Even with this challenge, we found no difference in the amount of cementum in mice lacking sclerostin compared with normal mice. Therefore, we conclude here that cementum is less sensitive to the absence of sclerostin compared with bone.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.24-0025","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T11:42:26Z","timestamp":1721130146000},"page":"82-96","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Disparate effects of sclerostin deletion on alveolar bone and cellular cementum in mice"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1848-0335","authenticated-orcid":false,"given":"Aonjittra","family":"Phanrungsuwan","sequence":"first","affiliation":[{"name":"Division of Biosciences College of Dentistry, The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0893-2139","authenticated-orcid":false,"given":"Michael B.","family":"Chavez","sequence":"additional","affiliation":[{"name":"Division of Biosciences College of Dentistry, The Ohio State University  Columbus Ohio USA"},{"name":"College of Dentistry, University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leena A.","family":"Eltilib","sequence":"additional","affiliation":[{"name":"Division of Biosciences College of Dentistry, The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara N.","family":"Kolli","sequence":"additional","affiliation":[{"name":"Division of Biosciences College of Dentistry, The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma F.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Division of Biosciences College of Dentistry, The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle H.","family":"Tan","sequence":"additional","affiliation":[{"name":"Division of Biosciences College of Dentistry, The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane R.","family":"Salmon","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Periodontics, Division of Periodontics Piracicaba Dental School, State University of Campinas  S\u00e3o Paulo Brazil"},{"name":"Faculty of Dentistry, N. 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