{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:26:36Z","timestamp":1789194396246,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Teeth and supporting oral tissues are attractive and accessible sources of stem cells. Periodontal ligament stem cells (PDLSC) are readily isolated from extracted third molars, and exhibit the ability to self\u2010renew and differentiate into multiple mesodermal cell fates. Clinical experience suggests that the exact location of periodontal defects affects the oral bone remodeling and wound healing. Compared to the mandible, the maxilla heals quicker and more efficiently. Angiogenesis is key in tissue regeneration including dental tissues, yet few studies focus on the angiogenic potential of PDLSC, none of which considered the differences between upper and lower jaw PDLSC (u\u2010PDLSC and l\u2010PDLSC, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here we studied the angiogenic potential of u\u2010PDLSC and l\u2010PDLSC and compared the results to well\u2010established mesenchymal stem cells (MSC). Cells were characterized in terms of surface markers, proliferation, and vascular endothelial growth factor (VEGF) secretion, and angiogenic assays were performed. Newly formed capillaries were stained with CD31, and their expression of platelet endothelial cell adhesion molecule (PECAM\u20101), angiopoietin 2 (ANGPT2), and vascular endothelial growth factor receptor 1 and 2 (VEGFR\u20101, VEGFR\u20102) were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Periodontal stem cells from the upper jaw showed a higher proliferation capacity, secreted more VEGF, and formed capillary networks faster and denser than l\u2010PDLSC. Gene expression of angiogenesis\u2010related genes was significantly higher in u\u2010PDLSC than in l\u2010PDLSC or MSC, given that culture conditions were suitable.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The oral cavity is a valuable source of stem cells, particularly PDLSC, which are promising for oral tissue engineering due to their robust growth, lifelong accessibility, low immunogenicity, and strong differentiation potential. Notably, u\u2010PDLSC exhibit higher VEGF secretion and accelerate capillary formation compared to l\u2010PDLSC or MSC. This study suggests a potential molecular mechanism in capillary formation, emphasizing the significance of precise location isolation of PDLSC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.24-0070","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T07:48:41Z","timestamp":1714981721000},"page":"662-672","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Angiogenic potential in periodontal stem cells from upper and lower jaw: A pilot study"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5992-510X","authenticated-orcid":false,"given":"Hanna","family":"Malyaran","sequence":"first","affiliation":[{"name":"Interdisciplinary Center for Clinical Research (IZKF) RWTH Aachen University  Aachen Germany"},{"name":"Helmholtz Institute for Biomedical Engineering BioInterface Group RWTH Aachen University  Aachen Germany"},{"name":"Department of Orthodontics University Hospital of RWTH Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Radermacher","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Biomedical Engineering BioInterface Group RWTH Aachen University  Aachen Germany"},{"name":"Department of Orthodontics University Hospital of RWTH Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogerio B.","family":"Craveiro","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University Hospital of RWTH Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P.","family":"K\u00fchnel","sequence":"additional","affiliation":[{"name":"Institute of Pathology RWTH Aachen University  Aachen Germany"},{"name":"Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) German Center for Lung Research (DZL)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny","family":"Jonigk","sequence":"additional","affiliation":[{"name":"Institute of Pathology RWTH Aachen University  Aachen Germany"},{"name":"Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) German Center for Lung Research (DZL)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University Hospital of RWTH Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Neuss","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Biomedical Engineering BioInterface Group RWTH Aachen University  Aachen Germany"},{"name":"Institute of Pathology RWTH Aachen University  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0512"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509340867"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910208100806"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00139.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2017.0004"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020929"},{"issue":"4","key":"e_1_2_10_9_1","first-page":"e526","article-title":"The role of angiogenesis in implant dentistry part II: the effect of bone\u2010grafting and barrier membrane materials on angiogenesis","volume":"21","author":"Saghiri M\u2010A","year":"2016","journal-title":"Medicina Oral, Patologia Oral Y Cirugia Bucal"},{"key":"e_1_2_10_10_1","volume-title":"Netter's Head and Neck Anatomy for Dentistry","author":"Norton","year":"2016"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18147447"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.22-0706"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5082\/6\/1\/015005"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0226"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1996.0142"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25063207"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(92)90364-3"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202003528"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.3c00269"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-67289-8"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldt031"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2014.03.008"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.240309797"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0562"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2015.2314"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2013.03.011"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma12132157"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/dj9010009"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2005.11.008"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509341637"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222212363"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010319\u201022345\u20102_5"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00524.2001"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.08.002"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034520902458"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2009.152"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v041a21"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/JPER.24-0070","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:09:16Z","timestamp":1760879356000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1002\/JPER.24-0070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,6]]},"references-count":37,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/JPER.24-0070"],"URL":"https:\/\/doi.org\/10.1002\/jper.24-0070","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,6]]},"assertion":[{"value":"2024-02-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}