{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:50:28Z","timestamp":1786978228517,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Recent findings have associated alveolar bone loss (ABL) with the presence of senescent cells. Aging and the accumulation of senescent cells are associated with a pro\u2010inflammatory environment which may exacerbate tissue damage.\u00a0Senolytics, such as dasatinib and quercetin (D&amp;Q), eliminate senescent cells, offering potential therapeutic benefits. We evaluated the effects of D&amp;Q on ABL during physiological aging and experimental periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study evaluated the effects of D&amp;Q in two in vivo models: aging and ligature\u2010induced periodontitis. In the aging model, 24 male C57BL\/6 mice were allocated to CTRL (control\u201030\u00a0weeks of age), AGED (vehicle\u2010treated, euthanized after 10 months), or D&amp;Q (monthly gavage of D&amp;Q [5\u00a0mg\/kg and 50\u00a0mg\/kg] for 10 months). In the periodontitis model, 24 Wistar rats (12\u00a0weeks old) were divided into CTRL, EP (experimental periodontitis, ligature +\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      ), or D&amp;Q (EP + weekly D&amp;Q at 5\u00a0mg\/kg and 50\u00a0mg\/kg). The ABL and bone microarchitecture were assessed by microcomputed tomography and histomorphometry, and gene expression was also analyzed. The microarchitecture of the femurs was evaluated for systemic effects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In aged mice, D&amp;Q reduced ABL, preserved the femoral cortical microarchitecture, and downregulated\n                      <jats:italic>Il6<\/jats:italic>\n                      ,\n                      <jats:italic>Mmp13<\/jats:italic>\n                      and\n                      <jats:italic>Lamb1<\/jats:italic>\n                      expression. In contrast, D&amp;Q failed to prevent ABL in the periodontitis model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>D&amp;Q treatment mitigated age\u2010related bone loss; but did not prevent EP associated bone loss under the therapeutic regimen used. These results suggest that the effects of senolytic therapy depend on disease context and treatment conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Plain language summary<\/jats:title>\n                    <jats:p>Aging increases the risk of periodontitis due to immune decline, chronic inflammation, and the accumulation of senescent cells, which release inflammatory factors that damage tissues. Senolytic drugs, such as dasatinib and quercetin (D&amp;Q), selectively eliminate senescent cells and have shown promise in age\u2010related diseases. In this study, we tested whether D&amp;Q could reduce alveolar bone loss associated with aging or experimental periodontitis. The D&amp;Q treatment protected aged mice by reducing bone loss, but it failed to prevent bone loss in the periodontitis model. These results suggest that the benefits of senolytic therapy may depend on disease context and treatment conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jper.70115","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T14:50:32Z","timestamp":1774536632000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dasatinib and quercetin prevent alveolar bone loss in aged mice"],"prefix":"10.1002","author":[{"given":"Luisa S.","family":"Battistelli","sequence":"first","affiliation":[{"name":"Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo State University (UNESP)  S\u00e3o Jos\u00e9 dos Campos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata M.","family":"Moraes","sequence":"additional","affiliation":[{"name":"Center for Pediatric Neurological Disease Research St. Jude Children's Research Hospital  Memphis Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri C.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo State University (UNESP)  S\u00e3o Jos\u00e9 dos Campos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6398-7880","authenticated-orcid":false,"given":"Jaqueline L.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo State University (UNESP)  S\u00e3o Jos\u00e9 dos Campos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u00edra T.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo State University (UNESP)  S\u00e3o Jos\u00e9 dos Campos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata F.","family":"Prado","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo State University (UNESP)  S\u00e3o Jos\u00e9 dos Campos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florent","family":"Elefteriou","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3930-4274","authenticated-orcid":false,"given":"Ana Lia","family":"Anbinder","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo State University (UNESP)  S\u00e3o Jos\u00e9 dos Campos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12732"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201061002\u20109"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40496\u2010018\u20100202\u20102"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12135"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/froh.2022.817249"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2019.030501"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.02.001"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207441"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2019.115220"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.20\u20100529"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12344"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2017.04.013"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16350"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4385"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_2_11_17_1","unstructured":"UrbaniakGC PlousS.Research Randomizer (Version 4.0) [computer software]. 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