{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:23:25Z","timestamp":1790713405793,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Periodontitis is an inflammatory disease of the oral cavity driven by bacterial dysbiosis that progressively breaks down the tooth\u2010supporting structures.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n This study leverages a uniquely large dataset of up to 34,000 patient records in the United States analyzed using standardized real\u2010time polymerase chain reaction\u2010based molecular testing to quantify bacterial profiles associated with periodontitis. Our analysis focused on 11 bacterial taxa from the red, orange, and green complexes. We assessed bacterial abundance, expressed as genome copies per milliliter, across periodontitis stages and associations with age and systemic conditions.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Across stages 1\u20134 (according to the American Academy of Periodontology classification), the concentration of the three red complex bacteria,\n Porphyromonas gingivalis<\/jats:italic>\n ,\n Tannerella forsythia<\/jats:italic>\n , and\n Treponema denticola<\/jats:italic>\n increased up to 11\u2010fold, the highest increase observed among all studied bacteria. A substantial microbial shift in younger patients was observed, while older individuals exhibited a higher prevalence of red complex pathogens. The relative abundance of\n P. gingivalis<\/jats:italic>\n increased significantly, from 4% in stage 1 periodontitis to 17% in stage 4. Cumulative bacterial load analysis based on the presence or absence of red complex bacteria revealed that the median bacterial load was 5\u2010fold higher in samples where red complex bacteria were present. The inter\u2010bacterial correlations increased with demographic and clinical variables as periodontitis advanced.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The findings revealed a significant positive association between increased bacterial loads, particularly red complex bacteria and higher periodontitis stages, positioning them as a key indicator of dysbiosis and a potential biomarker for disease advancement.<\/jats:p>\n <\/jats:sec>\n \n Plain language summary<\/jats:title>\n \n Periodontitis is a serious gum disease that damages the tissues supporting the teeth. It is caused by harmful bacteria in the mouth and becomes worse over time if not treated. In this study, we analyzed oral rinse samples collected from more than 34,000 people using a DNA\u2010based method to measure the levels of different bacteria. We focused on 11 bacteria known to be involved in gum disease and compared their presence in healthy versus mild to severe cases. We found that three bacteria,\n Porphyromonas gingivalis<\/jats:italic>\n ,\n Tannerella forsythia<\/jats:italic>\n , and\n Treponema denticola<\/jats:italic>\n , became much more common as the disease got worse, with one of them,\n P. gingivalis<\/jats:italic>\n , increasing 4\u2010fold in severe cases compared with mild ones. Older adults were more likely to have these harmful bacteria, but younger patients showed notable shifts in their bacterial makeup as well. These results suggest that certain bacteria, especially\n P. gingivalis<\/jats:italic>\n , may serve as warning signs for more advanced gum disease. Our findings could help to improve how gum disease is diagnosed and tracked, leading to better care and prevention. This is the largest study of its kind and offers new insight into the role of bacteria in oral health.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jper.70122","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:04:06Z","timestamp":1776085446000},"page":"1733-1745","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Correlating bacterial load and periodontal disease severity: A large\u2010scale study of the microbial landscape"],"prefix":"10.1002","volume":"97","author":[{"given":"Naomi B.","family":"Gizaw","sequence":"first","affiliation":[{"name":"Access Genetics & OralDNA Labs Eden Prairie Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Gomez","sequence":"additional","affiliation":[{"name":"Access Genetics & OralDNA Labs Eden Prairie Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald 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