{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T15:26:19Z","timestamp":1747149979117,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Patients with moderate\u2010severe Crohn's disease (CD) who are treated with antitumor necrosis factor alpha (TNF\u2010\u03b1) agents may be subjected to primary nonresponse or partial response. We aimed to identify tissue markers that may predict response to these agents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Pediatric patients (6\u201318 years) with either ileal or ileo\u2010colonic CD who were treated with anti\u2010TNF\u2010\u03b1 were stratified into three different groups based on their overall response to therapy at the end of induction including clinical and laboratory parameters (group 1\u2014full responders [FR], group 2\u2014partial responders [PR], group 3\u2014nonresponders [NR]). Seven tissue markers (fibronectin, interleukin [IL]\u201023R, IL\u201023, TNF\u2010\u03b1, collagen\u2010III, IL\u201013R, and hypoxia\u2010inducible factors [HIF]\u20101\u03b1) were evaluated. Immunofluorescence (IF) analyses were performed on biopsies from the terminal ileum, which were retrieved up to 6 months before treatment initiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010six CD patients (16 [61.5%] males; age 13.9\u2009\u00b1\u20092.9 years), including 8 (30.8%) with ileal disease and 18 (69.2%) with ileo\u2010colonic disease, were enrolled. Terminal ileum biopsies from nine patients from group 1, nine from group 2, and eight from group 3 were evaluated. Three antibodies were found to be significantly different between NR and FR groups; Collagen III and fibronectin stains were significantly more prominent in NR patients, while TNF\u2010\u03b1 stain was significantly more pronounced in FR, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 for each. PR could not have been predicted with neither of markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Decreased tissue IF intensity of fibronectin and collagen III and increased intensity of TNF\u2010\u03b1 may predict response to anti\u2010TNF\u2010\u03b1 treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jpn3.12146","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T09:23:25Z","timestamp":1706779405000},"page":"662-669","source":"Crossref","is-referenced-by-count":0,"title":["Tissue markers may predict treatment response to antitumor necrosis factor\u2010\u03b1 agents in children with Crohn's disease"],"prefix":"10.1002","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3120-7736","authenticated-orcid":false,"given":"Alexander","family":"Krauthammer","sequence":"first","affiliation":[{"name":"Schneider Children's Medical Center of Israel, Institute of Gastroenterology Nutrition and Liver Diseases Petah 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