{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:26:57Z","timestamp":1788272817836,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The latest European Society of Gastroenterology Hepatology and Nutrition (ESPGHAN) criteria for celiac disease (CD) diagnosis reduced the requirement for a small intestinal biopsy but still, for most of the cases a small intestinal biopsy is required for a safe diagnosis: hence the attempt to identify serum biomarkers that could replace, in most of these latter cases, the requirement of the biopsy (what is called a \u201cliquid biopsy\u201d). The aim of this study is to identify a set of serum biomarkers able to differentiate celiac patients from age and human leukocyte antigen (HLA)\u2010matched healthy controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Relative concentration of 92 inflammation\u2010linked proteins was examined in the sera of 50 children with CD compared with 50 HLA DQ2\/8 and age\u2010matched healthy controls born in genetically at\u2010risk families, using proximity extension immunoassay technology (Olink Proteomics\u00ae) with the ProSeek Multiplex Inflammation panel.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Three different multivariate analysis (Univariate and multivariate distribution analysis, random forest classification and linear discriminant analysis) localized a cluster of seven molecules (CASP8, CXCL9, NT\u20103, SIRT2, STAMBP, ST1A1, and TNFSF14) with a remarkable diagnostic potential, able to differentiate around 90% (95% confidence interval [CI]; 0.7\u20130.99) of CD patients from controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Patients with CD, compared to age\u2010 and HLA\u2010matched healthy controls, show in their sera an increased expression of inflammatory molecules, involved in NF\u2010\u03baB cytokine signaling, cell apoptosis, and crypt proliferation pathways. A set of seven of these proteins can differentiate cases from controls with an accuracy higher than 90%. The implementation of this approach in clinical setting could in future facilitate a noninvasive and individualized approach for CD diagnosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jpn3.70261","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T09:22:32Z","timestamp":1763630552000},"page":"508-515","source":"Crossref","is-referenced-by-count":1,"title":["A set of serum proteomic biomarkers differentiates celiac children from age and human leukocyte antigen\u2010matched healthy controls"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1806-0147","authenticated-orcid":false,"given":"Roberta","family":"Mandile","sequence":"first","affiliation":[{"name":"European Laboratory for Food Induced Disease (ELFID) University Federico II of Naples Naples Italy"},{"name":"Department of Translational Medical Sciences University Federico II of Naples Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele Francesco","family":"di Tolla","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences University Federico II of Naples Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Carpinelli","sequence":"additional","affiliation":[{"name":"European Laboratory for Food Induced Disease (ELFID) University Federico II of Naples Naples Italy"},{"name":"Department of Translational Medical Sciences University Federico II of Naples Naples Italy"},{"name":"Laboratory of Pediatrics, Division Gastroenterology and Nutrition Erasmus University Medical Centre \u2010 Sophia Children's Hospital, on behalf of the Dutch TIMID Consortium Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janneke Nicoline","family":"Samsom","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatrics, Division Gastroenterology and Nutrition Erasmus University Medical Centre \u2010 Sophia Children's Hospital, on behalf of the Dutch TIMID Consortium Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"De Cegli","sequence":"additional","affiliation":[{"name":"Telethon Institute of Genetics and Medicine TIGEM, Pozzuoli Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Vittoria","family":"Barone","sequence":"additional","affiliation":[{"name":"European Laboratory for Food Induced Disease (ELFID) University Federico II of Naples Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Troncone","sequence":"additional","affiliation":[{"name":"European Laboratory for Food Induced Disease (ELFID) University Federico II of Naples Naples Italy"},{"name":"Department of Translational Medical Sciences University Federico II of Naples Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Formisano","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences University Federico II of Naples Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Greco","sequence":"additional","affiliation":[{"name":"European Laboratory for Food Induced Disease (ELFID) University Federico II of Naples Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Auricchio","sequence":"additional","affiliation":[{"name":"European Laboratory for Food Induced Disease (ELFID) University Federico II of Naples Naples Italy"},{"name":"Department of Translational Medical Sciences University Federico II of Naples Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000002497"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios8020055"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.202100057"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202003526"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-008-0522-8"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.32383\/appdr\/157010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13369"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2024.09.001"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095192"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1000"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2024.03.042"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.05.013"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.665756"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2021.07.026"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.101093"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2010.276"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.04.023"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.06.002"}],"container-title":["Journal 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