{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T08:04:36Z","timestamp":1789200276384,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001858","name":"VINNOVA","doi-asserted-by":"publisher","award":["2019\u201001185"],"award-info":[{"award-number":["2019\u201001185"]}],"id":[{"id":"10.13039\/501100001858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Diagnostic delay is common in pediatric inflammatory bowel disease (PIBD), and fecal calprotectin (FCP) is often limited by challenges with sample collection. Therefore, we aimed to identify and validate a blood\u2010based diagnostic protein signature of PIBD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Proteins were analyzed using proximity extension assay in plasma samples from treatment\u2010na\u00efve pediatric patients in a Swedish inception cohort referred for suspected inflammatory bowel disease (IBD) and validated in an independent Norwegian population\u2010based pediatric inception cohort. Diagnostic performance was estimated by the area under the curve (AUC) with 95% confidence intervals (CIs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The discovery cohort included 58 patients with PIBD and 36 symptomatic controls without evidence of IBD, while the validation cohort consisted of 79 patients with PIBD and 37 symptomatic controls. In total, 154 proteins were examined. Univariable analyses identified 26 differentially regulated proteins for PIBD versus symptomatic controls in the discovery cohort (\n                      <jats:italic>q<\/jats:italic>\n                      \u2009&lt;\u20090.05), whereas 29 proteins were differentially regulated in the validation cohort. Using regularized logistic regression, we identified a diagnostic model of 31 proteins that differentiated PIBD from symptomatic controls in the discovery cohort (AUC\u2009=\u20090.83; 95% CI: 0.74\u20130.90). The protein signature was further reduced to a clinically relevant biomarker consisting of high\u2010sensitivity C\u2010reactive protein (hsCRP) and seven other proteins with diagnostic capacity (AUC\u2009=\u20090.85, 95% CI: 0.78\u20130.92) outperforming hsCRP in the validation cohort (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.006).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We identified and validated a blood\u2010based protein signature for PIBD with superior diagnostic performance compared to hsCRP. Given the challenges of fecal sample collection, further assay development may enable integration of these biomarkers into diagnostic pathways for PIBD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/ClinicalTrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      identifier: NCT02727959.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jpn3.70379","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T09:07:58Z","timestamp":1771232878000},"page":"1208-1217","source":"Crossref","is-referenced-by-count":1,"title":["A novel diagnostic serum protein signature for pediatric inflammatory bowel 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Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6990-0339","authenticated-orcid":false,"given":"Ove","family":"\u00d8y\u00e5s","sequence":"additional","affiliation":[{"name":"Oslo Centre for Biostatistics and Epidemiology Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1317-7422","authenticated-orcid":false,"given":"Manuela","family":"Zucknick","sequence":"additional","affiliation":[{"name":"Oslo Centre for Biostatistics and Epidemiology Oslo University Hospital Oslo Norway"},{"name":"Oslo Centre for Biostatistics and Epidemiology, Faculty of Medicine University of Oslo Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0283-2789","authenticated-orcid":false,"given":"Svend","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Vestfold Hospital trust 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