{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T20:44:00Z","timestamp":1760820240902},"reference-count":30,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pharmaceutical Sciences"],"published-print":{"date-parts":[[2010,12]]},"DOI":"10.1002\/jps.22199","type":"journal-article","created":{"date-parts":[[2010,6,22]],"date-time":"2010-06-22T18:22:16Z","timestamp":1277230936000},"page":"4866-4873","source":"Crossref","is-referenced-by-count":16,"title":["Subtilisin-\u03b3-Glutamyl Transpeptidase: A Novel Combination as Ungual Enhancer for Prospective Topical Application"],"prefix":"10.1016","volume":"99","author":[{"given":"Ekta","family":"Tiwary","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rani","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1002\/jps.22199_bb0005","first-page":"363","article-title":"Structural characteristics and permeability properties of the human nail: A review","volume":"50","author":"Gupchup","year":"1999","journal-title":"J Cosm Sci"},{"key":"10.1002\/jps.22199_bb0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-5173(01)00989-9","article-title":"Drug delivery to the nail following topical application","volume":"236","author":"Murdan","year":"2007","journal-title":"Int J Pharm"},{"key":"10.1002\/jps.22199_bb0015","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.ijpharm.2006.09.042","article-title":"An investigation into keratinolytic enzymes to enhance ungual drug delivery","volume":"332","author":"Mohorcic","year":"2007","journal-title":"Int J Pharam"},{"key":"10.1002\/jps.22199_bb0020","first-page":"696,164","article-title":"Antifungal treatement of nails","volume":"5","author":"Sun","year":"1997","journal-title":"US patent"},{"key":"10.1002\/jps.22199_bb0025","series-title":"Percutaneous absorption, drugs-cosmatics-mechanism-methodology, nail penetration","first-page":"759","article-title":"Focus on topical delivery of antifungal drugs for onchomycosis treatment","author":"Sun","year":"1999"},{"key":"10.1002\/jps.22199_bb0030","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0928-0987(97)00270-4","article-title":"Nail penetration of the antifungal agent oziconazole after repeated topical application in healthy volunteers and the effect of acetylcysteine","volume":"5","author":"Vanhoogdalem","year":"1997","journal-title":"Eur J Pharm Sci"},{"key":"10.1002\/jps.22199_bb0035","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1248\/cpb.46.1797","article-title":"Enhancing effect of N-acetyl-L-cysteine or 2-mercaptoethanol on the in vitro permeation of 5-fuorouracil or tolnaftate through human nail plate","volume":"46","author":"Kobayashi","year":"1998","journal-title":"Chem Pharm Bull"},{"key":"10.1002\/jps.22199_bb0040","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1002\/jps.10058","article-title":"Investigation of nail permeation enhancement by chemical modification using water as a probe","volume":"91","author":"Malhotra","year":"2002","journal-title":"J Pharm Sci"},{"key":"10.1002\/jps.22199_bb0045","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S1381-1177(02)00132-7","article-title":"Hydrolysis of native proteins by keratinolytic protease of Doratomyces microsporous","volume":"21","author":"Friedrich","year":"2003","journal-title":"J Mol Catal B: Enzyme"},{"key":"10.1002\/jps.22199_bb0050","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0378-5173(01)00749-9","article-title":"Comparison of two hard keratinuous substrates submitted to the action of a keratinase using an experimental design","volume":"224","author":"Vignardet","year":"2001","journal-title":"Int J Pharm"},{"key":"10.1002\/jps.22199_bb0055","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00253-005-0239-8","article-title":"Microbial keratinase and their prospective applications: An overview","volume":"70","author":"Gupta","year":"2006","journal-title":"Appl Micobiol Biotech-nol"},{"key":"10.1002\/jps.22199_bb0060","doi-asserted-by":"crossref","first-page":"20143","DOI":"10.1016\/S0021-9258(20)80705-4","article-title":"Recombinant canditropsin, an extracellular aspartic protease from yeast Candida tropicalis","volume":"268","author":"Lin","year":"1993","journal-title":"J Biol Chem"},{"key":"10.1002\/jps.22199_bb0065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0960-8524(98)00033-9","article-title":"A review: Potential of biotechnological applications of keratin degrading microorganisms and their enzymes for nutritional improvement of feathers and keratins as livestock feed resources","volume":"66","author":"Onifade","year":"1998","journal-title":"Biores Technol"},{"key":"10.1002\/jps.22199_bb0070","series-title":"Fungal biology","first-page":"326","article-title":"The mold of man","author":"Deacon","year":"2005"},{"key":"10.1002\/jps.22199_bb0075","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1046\/j.1365-2672.1999.00485.x","article-title":"Screening fungi for synthesis of keratinolytic enzymes","volume":"28","author":"Friedrich","year":"1999","journal-title":"Lett Appl Microbiol"},{"key":"10.1002\/jps.22199_bb0080","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1007\/s11274-007-9398-3","article-title":"Keratinase vis-\u00e0-vis conventional protease ad feather degradation","volume":"23","author":"Ramnani","year":"2007","journal-title":"World J Microbiol Biotechnol"},{"key":"10.1002\/jps.22199_bb0085","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1002\/jps.22199_bb0090","series-title":"Molecular cloning: A laboratory manual","author":"Sambrook","year":"2001"},{"key":"10.1002\/jps.22199_bb0095","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s00253-006-0440-4","article-title":"Overexpression, one step purification and biochemical characterization of recombinant \u03b3-glutamyl transpeptidase from Bacillus licheni-formis","volume":"73","author":"Lin","year":"2006","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1002\/jps.22199_bb0100","doi-asserted-by":"crossref","DOI":"10.1002\/jps.21743","article-title":"TranScreen-NTM: Method for rapid screening of trans-ungual drug delivery enhancers","volume":"98","author":"Murthy","year":"2009","journal-title":"J Pharm Sci"},{"key":"10.1002\/jps.22199_bb0105","doi-asserted-by":"crossref","first-page":"5169","DOI":"10.1128\/jb.171.9.5169-5172.1989","article-title":"DNA sequence of the Escherichia coli K-12 \u03b3-glutamyl transpeptidase gene ggt","volume":"171","author":"Suzuki","year":"1989","journal-title":"J Bacteriol"},{"key":"10.1002\/jps.22199_bb0110","doi-asserted-by":"crossref","first-page":"4319","DOI":"10.1128\/jb.178.14.4319-4322.1996","article-title":"Identification, sequence and expression of the gene encoding \u03b3-glutamyl transpeptidase in Bacillus subtilis","volume":"178","author":"Xu","year":"1996","journal-title":"J Bacteriol"},{"key":"10.1002\/jps.22199_bb0115","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1038\/sj.jim.2900667","article-title":"Fermentation production of keratinase from Bacillus licheniformis PWD-1 and a recombinant B. subtilis FDB-29","volume":"22","author":"Wang","year":"1999","journal-title":"J Ind Microbiol Biotechnol"},{"key":"10.1002\/jps.22199_bb0120","doi-asserted-by":"crossref","unstructured":"Gradi\u0161ar H, Friedrich J, Krizag I, Jerala R. Similarities and specificities of fungal keratinolytic protease: Keratinase of Paecilomyces marquandii and Doratomyces micrsporous in comparison to some known protease. Appl Environ Microbiol 71: 3420\u20133426.","DOI":"10.1128\/AEM.71.7.3420-3426.2005"},{"key":"10.1002\/jps.22199_bb0125","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1016\/S0006-291X(02)00580-6","article-title":"Keratin degradation: A cooperative action of two enzymes from Stenotrophomonas sp","volume":"294","author":"Yamamura","year":"2002","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1002\/jps.22199_bb0130","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1139\/w04-123","article-title":"Keratinolytic potential of Bacillus licheniformis RG1: Structural and biochemical mechanism of feather degradation","volume":"51","author":"Ramnani","year":"2005","journal-title":"Can J Microbiol"},{"key":"10.1002\/jps.22199_bb0135","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1038\/378416a0","article-title":"A protein catalytic framework with N-terminal nucleophile capable of self activation","volume":"378","author":"Brannigan","year":"1995","journal-title":"Nature (London)"},{"key":"10.1002\/jps.22199_bb0140","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/s00284-008-9250-5","article-title":"Influence of N-terminal truncation on the functional expression of Bacillus licheniformis \u03b3-glutamyl transpeptidase in recombinant Escherichia coil","volume":"57","author":"Lin","year":"2008","journal-title":"Curr Microbiol"},{"key":"10.1002\/jps.22199_bb0145","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1468-3083.2004.00988.x","article-title":"Topical antifungal drugs for the treatment of onychomycosis: An overview of current strategies for monotherapy and combination therapy","volume":"19","author":"Baran","year":"2005","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"10.1002\/jps.22199_bb0150","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0378-5173(99)00283-5","article-title":"A first order experimental design to access soluble proteins released by a new keratinase from Doratomyces microsporous on human substrates","volume":"191","author":"Vignardet","year":"1999","journal-title":"Int J Pharm"}],"container-title":["Journal of Pharmaceutical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022354915323753?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022354915323753?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T23:55:31Z","timestamp":1635551731000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022354915323753"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["S0022354915323753"],"URL":"https:\/\/doi.org\/10.1002\/jps.22199","relation":{},"ISSN":["0022-3549"],"issn-type":[{"value":"0022-3549","type":"print"}],"subject":[],"published":{"date-parts":[[2010,12]]}}}