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The two water\u2010insoluble solutes dissolved in pre\u2010formed one\u2010phase BMEs of Water\/Polysorbate 80\/Limonene\/Ethanol\/Glycerol and Water\/Aerosol\u2010OT (AOT)\/Polysorbate 85\/Isopropyl Myristate and an AOT\/Polysorbate 85 Winsor\u2010III system, achieving gramicidin and chlorhexidine concentrations of 1.0\u2009wt% and 0.5% individually and 0.5% and 0.3% in mixtures at 22\u00b0C, respectively. Small\u2010angle neutron scattering measurements demonstrated that both solutes decreased surfactant interfacial activity and increased interfacial fluidity for the Polysorbate 80 system. For the AOT\/Polysorbate 85 systems, ellipsoidal aggregates consisting of gramicidin and likely adsorbed surfactant and oil formed, while chlorhexidine enhanced the surface activity of surfactants. According to bioassays performed on artificial skin, the incorporation of melittin, gramicidin, and chlorhexidine in general enhanced the bioactivity of Polysorbate 80 BMEs for 24\u2009h treatment against relevant antibiotic\u2010resistant bacteria found on skin relative to controls. Yet, BME treatments were less effective than aqueous melittin control, in contrast to well diffusion bioassays performed previously. 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