{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:41:19Z","timestamp":1784828479699,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T00:00:00Z","timestamp":1573084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2020,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>In order to utilize tilapia skin gelatin hydrolysate protein, which is normally discarded as industrial waste in the process of fish manufacture, we study the <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> angiotensin\u2010I\u2010converting enzyme (ACE) inhibitory activity of the peptide Leu\u2010Ser\u2010Gly\u2010Tyr\u2010Gly\u2010Pro (LSGYGP). The aim was to provide a pharmacological basis of the development of minimal side effects of ACE inhibitors by comparative analysis with captopril in molecular docking.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>This peptide from protein\u2010rich wastes showed excellent ACE inhibitory activity (IC<jats:sub>50<\/jats:sub>\u00a0=\u00a02.577\u2009\u03bcmol\u2009L<jats:sup>\u22121<\/jats:sup>) and exhibited a mixed noncompetitive inhibitory pattern with Lineweaver\u2013Burk plots. Furthermore, LSGYGP and captopril groups both showed significant decreases in blood pressure after 6\u2009h and maintained good digestive stability over 4\u2009h. Molecular bond interactions differentiate competitive captopril upon hydrogen bond interactions and Zn(II) interaction. The C\u2010terminal Pro generates three interactions (hydrogen bonds, hydrophilic interactions and Van der Waals interactions) in the peptide and effectively interacts with the S1 and S2 pockets of ACE.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>LSGYGP, with an IC<jats:sub>50<\/jats:sub> value of 2.577\u2009\u03bcmol\u2009L<jats:sup>\u22121<\/jats:sup>, has an antihypertensive effect in spontaneously hypertensive rats. Through comparison with captopril, this study revealed that LSGYGP may be a potential food\u2010derived ACE inhibitory peptide and could act as a functional food ingredient to prevent hypertension. \u00a9 2019 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10041","type":"journal-article","created":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T16:09:21Z","timestamp":1568650161000},"page":"315-324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Comparison of an angiotensin\u2010I\u2010converting enzyme inhibitory peptide from tilapia (<i>Oreochromis niloticus<\/i>) with captopril: inhibition kinetics, <i>in vivo<\/i> effect, simulated gastrointestinal digestion and a molecular docking study"],"prefix":"10.1002","volume":"100","author":[{"given":"Jiali","family":"Chen","sequence":"first","affiliation":[{"name":"College of Food Science and Technology Guangdong Ocean University  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