{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:38:38Z","timestamp":1789389518304,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T00:00:00Z","timestamp":1579910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31901688"],"award-info":[{"award-number":["31901688"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2020,3,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Sea cucumber is a rich source of eicosapentaenoic acid in the form of eicosapentaenoic acid\u2010enriched phospholipids (EPA\u2010PL). It is known to be efficacious in preventing obesity. However, few studies have focused on the role of EPA\u2010PL in inhibiting lipid accumulation by lipid droplets (LDs). This study first investigated the effect of EPA\u2010PL from sea cucumber on the formation of LDs and the underlying mechanism in C57BL\/6J mice. The mice were randomly divided into two groups and treated for 8\u2009weeks or 3, 7, and 14\u2009days with either (i) a high\u2010sucrose diet (model group), (ii) a high\u2010sucrose diet plus 2% EPA\u2010PL (EPA\u2010PL group).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Eight\u2010week EPA\u2010PL supplementation significantly reduced lipid accumulation and LD size in liver and white adipose tissue (WAT), which was accompanied by the decreased expression of LDs\u2010associated protein FSP27. A 3\u2010day EPA\u2010PL treatment suppressed the mRNA expression of <jats:italic>Fsp27<\/jats:italic>. The mRNA level of <jats:italic>Fsp27<\/jats:italic> reached its \u2018normal level\u2019 after withdrawing EPA\u2010PL for 7\u2009days, suggesting that EPA\u2010PL might serve as a rapid regulator of FSP27. Furthermore, EPA\u2010PL increased the expression of lipolysis genes <jats:italic>Hsl<\/jats:italic> and <jats:italic>Atgl<\/jats:italic> accompanied by the regulation of <jats:italic>Ppar\u03b3<\/jats:italic> in WAT.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Dietary EPA\u2010PL from sea cucumber (<jats:styled-content style=\"fixed-case\"><jats:italic>Cucumaria frondosa<\/jats:italic>)<\/jats:styled-content> protected against lipid accumulation by regulating LDs\u2010associated protein FSP27, which might provide novel evidence for the anti\u2010obesity action of EPA\u2010PL. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10250","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T21:15:43Z","timestamp":1578604543000},"page":"2244-2251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Eicosapentaenoic acid\u2010enriched phospholipids suppressed lipid accumulation by specific inhibition of lipid droplet\u2010associated protein FSP27 in mice"],"prefix":"10.1002","volume":"100","author":[{"given":"Lingyu","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haohao","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhu","family":"Xue","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao China"},{"name":"Qingdao National Laboratory for Marine Science and Technology Laboratory of Marine Drugs &amp; Biological Products  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5162-0408","authenticated-orcid":false,"given":"Tiantian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2310-1456","authenticated-orcid":false,"given":"Yuming","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao China"},{"name":"Qingdao National Laboratory for Marine Science and Technology Laboratory of Marine Drugs &amp; Biological Products  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.32"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2008.05.009"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-061009-102430"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-789X.2007.00311.x"},{"key":"e_1_2_6_6_1","first-page":"138","article-title":"Obesity and diabetes","volume":"28","author":"Riobo Servan P","year":"2013","journal-title":"Nutr Hosp"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.12.012"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.11.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0085-z"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2017.06.009"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.111.241489"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2014.03.008"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708323200"},{"key":"e_1_2_6_14_1","first-page":"2808","article-title":"FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets","volume":"118","author":"Nishino N","year":"2008","journal-title":"J Clin Invest"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M021899"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2008.10.005"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2013.10.015"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2016.06.014"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejlt.201300407"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2016.04.034"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2016.10.022"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b00810"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/82630"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.10.109"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2008.03.003"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.01.113"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002890"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27607"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8315454"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00100.2011"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2018.02.003"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2018.02.006"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M028472"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.539890"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6949"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.10250","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.10250","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.10250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:00:54Z","timestamp":1760871654000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.10250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,25]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,3,30]]}},"alternative-id":["10.1002\/jsfa.10250"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.10250","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,25]]},"assertion":[{"value":"2019-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}