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However, ginkgolic acid, one of the major components found in <jats:styled-content style=\"fixed-case\"><jats:italic>G. biloba<\/jats:italic><\/jats:styled-content> extract, may cause serious allergic and toxic side effects. The purpose of this study is to immobilize the laccase system on the electrospun nylon fiber mat (NFM) to hydrolyze the ginkgolic acid in <jats:styled-content style=\"fixed-case\"><jats:italic>G. biloba<\/jats:italic><\/jats:styled-content> leaf extract efficiently.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Novel electrospinning technology successfully produced high\u2010quality nanoscopic fiber mats made of a mixture of multi\u2010walled carbon nanotube and nylon 6,6. Laccase that was immobilized onto the NFM exhibited much higher efficiency in the catalyzation of 2,2\u2032\u2010azino\u2010bis(3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid) diammonium salt (ABTS) than nylon 6,6 pellets. After being immobilized onto the NFM, the pH and temperature stability of laccase were significantly improved. The NFM\u2010immobilized laccase could maintain more than 50% of its original activity even after 40\u2009days of storage or 10 operational cycles. The kinetic parameters, including rate constant (<jats:italic>K<\/jats:italic>), the time (\u03c450) in which 50% of ginkgolic acid hydrolysis was reached, the time (\u03c4complete) required to achieve complete ginkgolic acid hydrolysis, <jats:italic>K<\/jats:italic>m and Vmax were determined, and were 0.07\u2009\u00b1\u20090.01\u2009min<jats:sup>\u22121<\/jats:sup>, 8.97\u2009\u00b1\u20090.55\u2009min, 45.45\u2009\u00b1\u20092.79\u2009min, 0.51\u2009\u00b1\u20090.09\u2009mM and 0.49\u2009\u00b1\u20090.03\u2009mM\u2009min<jats:sup>\u22121<\/jats:sup>\u2009mg<jats:sup>\u22121<\/jats:sup>, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The result successfully demonstrated the strong potential of using novel electrospun nanofiber mats as enzyme immobilization platforms, which could significantly enhance enzyme activity and stability. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10301","type":"journal-article","created":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T00:14:07Z","timestamp":1580429647000},"page":"2705-2712","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Enzymatic degradation of ginkgolic acid by laccase immobilized on novel electrospun nanofiber mat"],"prefix":"10.1002","volume":"100","author":[{"given":"Hung\u2010Yueh","family":"Chen","sequence":"first","affiliation":[{"name":"Graduate Institute of Food Science Technology National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0387-7804","authenticated-orcid":false,"given":"Kuan\u2010Chen","family":"Cheng","sequence":"additional","affiliation":[{"name":"Graduate Institute of Food Science Technology National Taiwan University  Taipei Taiwan"},{"name":"Institute of Biotechnology 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