{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T09:46:50Z","timestamp":1785750410485,"version":"3.56.0"},"reference-count":46,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>It is known that cholecystokinin (CCK) plays an essential role in reducing food intake and driving weight loss. Previous studies demonstrated that amino acids were capable of triggering CCK release through G protein\u2010coupled receptors, but the sensing mechanism remains obscure, especially the intracellular signaling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:sc>l<\/jats:sc>\u2010Glu, rather than its <jats:sc>d<\/jats:sc>\u2010isomer, robustly stimulated CCK secretion in a porcine duodenal model, and the secretory response was augmented by incubation with the allosteric ligand of T1R1, while T1R3 antagonist attenuated it. Upon inhibiting phospholipase C (PLC) or transient receptor potential M5 (TRPM5) activity, <jats:sc>l\u2010<\/jats:sc>Glu failed to increase CCK release. Oral administration of monosodium glutamate in rats also suppressed food intake and increased plasma CCK levels, accompanied by elevated expression of <jats:italic>T1R1<\/jats:italic>, <jats:italic>PLC\u03b22<\/jats:italic> and <jats:italic>TRPM5<\/jats:italic> in the duodenum.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These data demonstrated that <jats:sc>l\u2010<\/jats:sc>Glu stimulated CCK secretion through the activation of T1R1\/T1R3 in a PLC\/TRPM5\u2010dependent manner. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10541","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T07:05:36Z","timestamp":1590995136000},"page":"4818-4825","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>l<\/scp>\u2010Glutamate stimulates cholecystokinin secretion via the <scp>T1R1<\/scp>\/<scp>T1R3<\/scp> mediated 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