{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T20:58:19Z","timestamp":1768683499674,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T00:00:00Z","timestamp":1595808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["ASTIP\u2010IAS07"],"award-info":[{"award-number":["ASTIP\u2010IAS07"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The present study investigated the chronic effect on the composition and proportions of the cecal microbiota of laying hens for 12\u2009weeks after consuming two genetically modified (GM) corns containing the <jats:italic>maroACC<\/jats:italic> gene from the <jats:italic>Agrobacterium tumefaciens<\/jats:italic> strain (CC) and the <jats:italic>mCry1Ac<\/jats:italic> gene from the <jats:italic>Bacillus thuringiensis<\/jats:italic> strain (BT) in comparison with the isogenic corn (CT).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In total, 72 hens were randomly assigned to the CT corn\u2010based diet, CC corn\u2010based diet and BT corn\u2010based diet. The absolute weights of abdominal fat, breast, thigh meat and organ weight were not affected by the dietary treatment. High\u2010throughput 16S rRNA gene sequencing revealed a few differences in the composition of cecal microbiota among the treatments. The only difference with respect to bacterial family was that the cecal abundance of <jats:italic>Porphyromonadaceae<\/jats:italic> (3.46 <jats:italic>versus<\/jats:italic> 2.11%; <jats:italic>P<\/jats:italic> = 0.073) tended to be higher for birds consuming the CC diet than those birds consuming the CT diet. Birds fed the BT diet tended to have a higher abundance of <jats:italic>Barnesiella<\/jats:italic> (0.62 <jats:italic>versus<\/jats:italic> 0.13%; <jats:italic>P<\/jats:italic> = 0.057) and a lower abundance of <jats:italic>unclassified Ruminococcaceae<\/jats:italic> (0.64 <jats:italic>versus<\/jats:italic> 1.19%; <jats:italic>P<\/jats:italic> = 0.097) than those fed the CT diet. Considering beneficial intestinal <jats:italic>Barnesiella<\/jats:italic>, this decreases and ultimately clears the colonization of vancomycin\u2010resistant <jats:italic>Enterococcus<\/jats:italic>. The <jats:italic>unclassified Ruminococcaceae<\/jats:italic> was a low\u2010frequency and low\u2010abundance bacterial taxa and was not associated with intestinal pathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These results indicate a similar modulation of cecal microbiota in laying hens by long\u2010term feeding among transgenic CC corn, BT corn and non\u2010transgenic corn and provide data for biosafety evaluation of the transgenic corn. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10596","type":"journal-article","created":{"date-parts":[[2020,6,20]],"date-time":"2020-06-20T03:41:20Z","timestamp":1592624480000},"page":"5450-5457","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the <scp>maroACC<\/scp> or <scp>mCry1Ac<\/scp> genes"],"prefix":"10.1002","volume":"100","author":[{"given":"Ruqing","family":"Zhong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Sciences Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Sciences Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8748-0515","authenticated-orcid":false,"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Sciences Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoguang","family":"Yang","sequence":"additional","affiliation":[{"name":"National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongfu","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Sciences Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,27]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"ISAAA.2018.Global Status of Commercialized Biotech\/GM Crops in 2018: Biotech crops continue to help meet the challenges of increased population and climate change. 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