{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:33:57Z","timestamp":1788521637296,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya","doi-asserted-by":"publisher","award":["SGR 1008"],"award-info":[{"award-number":["SGR 1008"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Seafood parasitation by <jats:italic>Anisakis<\/jats:italic> (Anisakidae) larvae has been reported in most of the oceans and seas worldwide. The presence of these nematodes in commonly consumed fish represents a potential hazard for consumers as they can provoke gastrointestinal symptoms and allergic reactions. In the present work, the capacity of a SYBR Green qPCR protocol to quantify <jats:italic>Anisakis<\/jats:italic> larvae in commercial fish was evaluated using experimentally spiked samples with different numbers (0\u201350) of <jats:italic>A. simplex<\/jats:italic> third\u2010stage larvae (L3). To verify the agreement of the obtained results, 25 naturally infected fish specimens of Atlantic blue whiting underwent a parallel visual inspection.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The logarithmic behavior of the Cq data obtained from the experimentally spiked samples allowed the development of a descriptive mathematical model that correlates the Cq value with the number of <jats:italic>Anisakis<\/jats:italic> larvae (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> = 0.9908, CV = 2.37%). In the commercial blue whiting specimens there was a high correlation between the results of the molecular technique and the visual inspection (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> = 0.9912); the Bland\u2013Altman analysis showed that 94% of the differences were within the limits of agreement (\u22124.98 and 6.68), indicating the reliability of the descriptive mathematical model based on the SYBR Green qPCR technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The descriptive function presented based on the SYBR Green qPCR assay is promising as a sensitive and accurate tool for measuring the <jats:italic>Anisakis<\/jats:italic> larval load in commercial fish, with a potential application not only in the food industry but also in prevention programs for public health. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10718","type":"journal-article","created":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T09:51:55Z","timestamp":1596880315000},"page":"1085-1090","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Estimation of the number of <i>Anisakis<\/i> larvae in commercial fish using a descriptive model based on real\u2010time <scp>PCR<\/scp>"],"prefix":"10.1002","volume":"101","author":[{"given":"Carla","family":"God\u00ednez\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Laboratory of Parasitology, Department of Biology, Health and Environment, Faculty of Pharmacy and Food Sciences University of Barcelona  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Roca\u2010Geron\u00e8s","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Department of Biology, Health and Environment, Faculty 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