{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:39:16Z","timestamp":1791646756178,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T00:00:00Z","timestamp":1599609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011698","name":"Junta de Comunidades de Castilla-La Mancha","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011698","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2021,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Oil mills could benefit by preparing their own aqueous extracts from olive leaves. Accordingly, the present study aimed to measure the bioactive compounds richness of such extracts, especially oleuropein. A water\u2010based microwave extraction procedure was developed and a selective and precise high\u2010performance liquid chromatography with diode array detection (HPLC\u2010DAD) method was validated for the determination of oleuropein and others bioactive compounds from olive leaves.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The water solubility of oleuropein was determined to be 9.5 g\u2009L<jats:sup>\u22121<\/jats:sup>. The extraction procedure was optimized in terms of power, olive leaf weight\/water volume ratio and time of extraction, and the results revealed that 2 mg\u2009mL<jats:sup>\u22121<\/jats:sup> and a microwave irradiation at 800\u2009W for 30\u2009s resulted in the greatest efficiency. Oleuropein was determined by the new validation method, which showed good linearity (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> = 0.996), precision (% relative standard deviation\u2009&lt;\u200910%), recovery (118.6%), and limits of detection (17.48\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup>) and quantification (21.54\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup>). Good correlation (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> = 0.979) was obtained between oleuropein of the olive leaf extracts determined by HPLC\u2010DAD and by UV\u2010visible spectrophotometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>A simple extraction method was developed and validated to obtain aqueous extract from olive leaves by microwave extraction, determining for the first time oleuropein water solubility. Validation of the method showed that oleuropein in olive leaves could be quantified when it is at least 1% of dry weight by means of HPLC\u2010DAD. UV\u2010visible spectrophotometry can be useful in oil mills because it enables the content of oleuropein and other bioactive compounds content to be determined <jats:italic>in situ<\/jats:italic> in such leaf aqueous extracts. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10758","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T08:01:15Z","timestamp":1598342475000},"page":"1447-1453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Development and validation of an <scp>HPLC\u2010DAD<\/scp> method for determination of oleuropein and other bioactive compounds in olive leaf by\u2010products"],"prefix":"10.1002","volume":"101","author":[{"given":"Esther M","family":"Mart\u00ednez\u2010Navarro","sequence":"first","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Agr\u00edcola Universidad de Castilla\u2010La Mancha, E.T.S.I. Agr\u00f3nomos y Montes  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Cebri\u00e1n\u2010Taranc\u00f3n","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Agr\u00edcola Universidad de Castilla\u2010La Mancha, E.T.S.I. Agr\u00f3nomos y Montes  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Moratalla\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Agr\u00edcola Universidad de Castilla\u2010La Mancha, E.T.S.I. Agr\u00f3nomos y Montes  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e1ndida","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Agr\u00edcola Universidad de Castilla\u2010La Mancha, E.T.S.I. Agr\u00f3nomos y Montes  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9980-5073","authenticated-orcid":false,"given":"Gonzalo L","family":"Alonso","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Agr\u00edcola Universidad de Castilla\u2010La Mancha, E.T.S.I. Agr\u00f3nomos y Montes  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0718-6410","authenticated-orcid":false,"given":"Rosario M","family":"Salinas","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Agr\u00edcola Universidad de Castilla\u2010La Mancha, E.T.S.I. 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